細胞視黃酸結合蛋白Ⅱ、表皮型脂肪酸結合蛋白及Ki-67在乳腺浸潤性導管癌中的表達及相關性_《華西醫學》

作者：

孟紅 ¹ , 劉倩 ¹ , 吳寧 ¹ , 蔣雪梅 ² , 何小艷 ¹ , 丁兀兀 ¹

德陽市人民醫院（四川德陽，618000）1 病理科，2乳腺外科;

關鍵詞：

乳腺腫瘤浸潤性導管癌細胞視黃酸結合蛋白Ⅱ 表皮型脂肪酸結合蛋白 Ki-67抗原

DOI：

10.7507/1002-0179.20130444

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的　研究細胞視黃酸結合蛋白（CRABP）Ⅱ、表皮型脂肪酸結合蛋白（E-FABP）和Ki-67在乳腺浸潤性導管癌中的表達情況及三者的相關性。方法　采用免疫組織化學檢測2001年1月－2007年12月手術切除的152例乳腺浸潤性導管癌中CRABPⅡ、E-FABP和Ki-67的表達。結果　在浸潤性導管癌中，CRABPⅡ在Ki-67陰性組的陽性率高于Ki-67陽性組（P＜0.05），相反地，E-FABP在Ki-67陽性組的陽性率高于Ki-67陰性組（P＜0.05）。CRABPⅡ和Ki-67表達呈負相關（rS=?0.432，P＜0.05）；E-FABP和Ki-67表達呈正相關（rS=0.842， P＜0.05）。E-FABP和Ki-67的表達具有協同性，E-FABP和Ki-67共同表達與腫瘤的轉移有關（P＜0.05）。單因素生存分析顯示，E-FABP的陽性表達患者、Ki-67的陽性表達患者以及E-FABP和Ki-67的共同陽性表達患者的預后差（P＜0.05）。多因素生存分析提示E-FABP的表達（RR=4.223，P=0.012）和TNM分期（RR=8.412，P=0.000）是影響浸潤性導管癌患者預后的獨立危險因素。結論　在乳腺浸潤性導管癌中，CRABPⅡ和E-FABP與腫瘤細胞的增殖有關，CRABPⅡ抑制細胞增殖，E-FABP促進細胞增殖。E-FABP和Ki-67在浸潤性導管癌的發生、發展中起協同作用，兩者的陽性表達可能對評估腫瘤的轉移和患者的預后有一定價值。

引用本文： 孟紅,劉倩,吳寧,蔣雪梅,何小艷,丁兀兀. 細胞視黃酸結合蛋白Ⅱ、表皮型脂肪酸結合蛋白及Ki-67在乳腺浸潤性導管癌中的表達及相關性. 華西醫學, 2013, 28(9): 1415-1419. doi: 10.7507/1002-0179.20130444 復制

1.	Schug TT, Berry DC, Shaw NS, et al. Opposing effects of retinoic acid on cell growth result from alternate activation of two different nuclear receptors[J]. Cell, 2007, 129(4): 723-733.
2.	Rochette-Egly C, Chambon P. F9 embryocarcinoma cells: a cell autonomous model to study the functional selectivity of RARs and RXRs in retinoid signaling[J]. Histol Histopathol, 2001, 16(3): 909-922.
3.	Donato LJ, Suh JH, Noy N. Suppression of mammary carcinoma cell growth by retinoic acid: the cell cycle control gene Btg2 is a direct target for retinoic acid receptor signaling[J]. Cancer Res, 2007, 67(2): 609-615.
4.	Donato LJ, Noy N. Suppression of mammary carcinoma growth by retinoic acid: proapoptotic genes are targets for retinoic acid receptor and cellular retinoic acid-binding proteinⅡ signaling[J]. Cancer Res, 2005, 65(18): 8193-8199.
5.	Di-Poi N, Tan NS, Michalik L, et al. Antiapoptotic role of PPAR beta in keratinocytes via transcriptional control of the akt1 signaling pathway[J]. Mol Cell, 2002, 10(4): 721-733.
6.	Vo HP, Crowe DL. Transcriptional regulation of retinoic acid responsive genes by cellular retinoic acid binding protein-Ⅱ modulates RA mediated tumor cell proliferation and invasion[J]. Anticancer Res, 1998, 18(1A): 217-224.
7.	Budhu AS, Noy N. Direct channeling of retinoic acid between cellular retinoic acid-binding proteinⅡ and retinoic acid receptor sensitizes mammary carcinoma cells to retinoic acid-induced growth arrest[J]. Mol Cell Biol, 2002, 22(8): 2632-2641.
8.	Manor D, Shmidt EN, Budhu A, et al. Mammary carcinoma suppression by cellular retinoic acid binding protein-Ⅱ[J]. Cancer Res, 2003, 63(15): 4426-4433.
9.	湯參娥, 李萃, 肖志強, 等. 人肺鱗癌組織的比較蛋白質組學研究[J]. 中華腫瘤雜志, 2006, 28(4): 274-279.
10.	Fuj K, Kondo T, Yokoo H, et al. Proteomic study of human hepatocellular carcinoma using two-dimensional difference gel electrophoresis with saturation cysteine dye[J]. Proteomics, 2005, 5(5): 1411-1422.
11.	Adamson J, Morgan EA, Beesley C, et al. High-level expression of cutaneous fatty acid-binding protein in prostatic carcinomas and its effect on tumorigenicity[J]. Oncogene, 2003, 22(18): 2739-2749.
12.	Jing C, Beesley C, Foster CS, et al. Identification of the messenger RNA for human cutaneous fatty acid-binding protein as a metastasis inducer[J]. Cancer Res, 2000, 60(9): 2390-2398.
13.	Jing C, Beesley C, Foster CS, et al. Human cutaneous fatty acid-binding protein induces metastasis by up-regulating the expression of vascular endothelial growth factor gene in rat Rama 37 model cells[J]. Cancer Res, 2001, 61(11): 4357-4364.
14.	Bouzubar N, Walker KJ, Griffiths K, et al. Ki-67 immunostaining in primary breast cancer: pathological and clinical associations[J]. Br J Cancer, 1989, 59(6): 943-947.
15.	Goldhirsch A, Ingle JN, Gelber RD, et al. Thresholds for therapies: highlights of the St Gallen International Expert Consensus on the primary therapy of early breast cancer 2009[J]. Ann Oncol, 2009, 20(8): 1319-1329.
16.	唐平, 魏兵, 楊雯娟, 等. 乳腺癌預后/預測因子[J]. 中華病理學雜志, 2011, 40(2): 73-76.

1. 　Schug TT, Berry DC, Shaw NS, et al. Opposing effects of retinoic acid on cell growth result from alternate activation of two different nuclear receptors[J]. Cell, 2007, 129(4): 723-733.
2. 　Rochette-Egly C, Chambon P. F9 embryocarcinoma cells: a cell autonomous model to study the functional selectivity of RARs and RXRs in retinoid signaling[J]. Histol Histopathol, 2001, 16(3): 909-922.
3. 　Donato LJ, Suh JH, Noy N. Suppression of mammary carcinoma cell growth by retinoic acid: the cell cycle control gene Btg2 is a direct target for retinoic acid receptor signaling[J]. Cancer Res, 2007, 67(2): 609-615.
4. 　Donato LJ, Noy N. Suppression of mammary carcinoma growth by retinoic acid: proapoptotic genes are targets for retinoic acid receptor and cellular retinoic acid-binding proteinⅡ signaling[J]. Cancer Res, 2005, 65(18): 8193-8199.
5. 　Di-Poi N, Tan NS, Michalik L, et al. Antiapoptotic role of PPAR beta in keratinocytes via transcriptional control of the akt1 signaling pathway[J]. Mol Cell, 2002, 10(4): 721-733.
6. 　Vo HP, Crowe DL. Transcriptional regulation of retinoic acid responsive genes by cellular retinoic acid binding protein-Ⅱ modulates RA mediated tumor cell proliferation and invasion[J]. Anticancer Res, 1998, 18(1A): 217-224.
7. 　Budhu AS, Noy N. Direct channeling of retinoic acid between cellular retinoic acid-binding proteinⅡ and retinoic acid receptor sensitizes mammary carcinoma cells to retinoic acid-induced growth arrest[J]. Mol Cell Biol, 2002, 22(8): 2632-2641.
8. 　Manor D, Shmidt EN, Budhu A, et al. Mammary carcinoma suppression by cellular retinoic acid binding protein-Ⅱ[J]. Cancer Res, 2003, 63(15): 4426-4433.
9. 　湯參娥, 李萃, 肖志強, 等. 人肺鱗癌組織的比較蛋白質組學研究[J]. 中華腫瘤雜志, 2006, 28(4): 274-279.
10. 　Fuj K, Kondo T, Yokoo H, et al. Proteomic study of human hepatocellular carcinoma using two-dimensional difference gel electrophoresis with saturation cysteine dye[J]. Proteomics, 2005, 5(5): 1411-1422.
11. 　Adamson J, Morgan EA, Beesley C, et al. High-level expression of cutaneous fatty acid-binding protein in prostatic carcinomas and its effect on tumorigenicity[J]. Oncogene, 2003, 22(18): 2739-2749.
12. 　Jing C, Beesley C, Foster CS, et al. Identification of the messenger RNA for human cutaneous fatty acid-binding protein as a metastasis inducer[J]. Cancer Res, 2000, 60(9): 2390-2398.
13. 　Jing C, Beesley C, Foster CS, et al. Human cutaneous fatty acid-binding protein induces metastasis by up-regulating the expression of vascular endothelial growth factor gene in rat Rama 37 model cells[J]. Cancer Res, 2001, 61(11): 4357-4364.
14. 　Bouzubar N, Walker KJ, Griffiths K, et al. Ki-67 immunostaining in primary breast cancer: pathological and clinical associations[J]. Br J Cancer, 1989, 59(6): 943-947.
15. 　Goldhirsch A, Ingle JN, Gelber RD, et al. Thresholds for therapies: highlights of the St Gallen International Expert Consensus on the primary therapy of early breast cancer 2009[J]. Ann Oncol, 2009, 20(8): 1319-1329.
16. 　唐平, 魏兵, 楊雯娟, 等. 乳腺癌預后/預測因子[J]. 中華病理學雜志, 2011, 40(2): 73-76.

《華西醫學》

細胞視黃酸結合蛋白Ⅱ、表皮型脂肪酸結合蛋白及Ki-67在乳腺浸潤性導管癌中的表達及相關性

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

上一篇

下一篇

Format

Content

《華西醫學》

細胞視黃酸結合蛋白Ⅱ、表皮型脂肪酸結合蛋白及Ki-67在乳腺浸潤性導管癌中的表達及相關性

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

上一篇

下一篇

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料