促進靜電紡絲支架中細胞滲透生長的研究進展_《中國修復重建外科雜志》

作者：

安博 , 孫銘澤 , 孫明林

武警后勤學院附屬醫院骨二科（天津，300162）;

關鍵詞：

組織工程靜電紡絲技術支架材料滲透生長

DOI：

10.7507/1002-1892.20130047

視頻：

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目的綜述改進靜電紡絲（簡稱電紡）技術促進支架內細胞滲透生長的研究進展。方法查閱近年有關改進電紡技術促進支架內細胞滲透生長的相關文獻，對相關技術方法和促進支架內細胞滲透生長的效果進行回顧及綜合分析。結果改進方法包括電紡參數調節、電紡支架加工修飾、細胞-支架復合物動態培養和其他方法等，但效果有限，仍需進一步優化。結論細胞在支架中的滲透生長在組織工程研究中意義重大，電紡技術應用瓶頸在于其致密排列的纖維和過小的腔隙孔徑，限制了支架內細胞的滲透生長，多種改進技術的聯合應用將是解決這一難題的方法。

引用本文： 安博,孫銘澤,孫明林. 促進靜電紡絲支架中細胞滲透生長的研究進展. 中國修復重建外科雜志, 2013, 27(2): 219-222. doi: 10.7507/1002-1892.20130047 復制

1.	Agarwal S, Wendorff JH, Greiner A. Progress in the field of electrospinning for tissue engineering applications. Adv Mater, 2009, 21(32-33): 3343-3351.
2.	胡旭棟, 王光林. 靜電紡絲納米纖維支架在神經組織工程中的研究進展. 中國修復重建外科雜志, 2010, 24(9): 1133-1137.
3.	薛正翔, 李敏. 血管組織工程支架研究進展. 中國修復重建外科雜志, 2009, 23(9): 1134-1137.
4.	Bhardwaj N, Kundu SC. Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv, 2010, 28(3): 325-347.
5.	Rnjak-Kovacina J, Weiss AS. Increasing the pore size of electrospun scaffolds. Tissue Eng Part B Rev, 2011, 17(5): 365-372.
6.	Liao S, Li B, Ma Z, et al. Biomimetic electrospun nanofibers for tissue regeneration. Biomed Mater, 2006, 1(3): R45-53.
7.	Li M, Mondrinos MJ, Gandhi MR, et al. Electrospun protein fibers as matrices for tissue engineering. Biomaterials, 2005, 26(30): 5999-6008.
8.	Eichhorn SJ, Sampson WW. Statistical geometry of pores and statistics of porous nanofibrous assemblies. J R Soc Interface, 2005, 2(4): 300-318.
9.	Pham QP, Sharma U, Mikos AG. Electrospun poly (epsilon-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: characterization of scaffolds and measurement of cellular infiltration. Biomacromolecules, 2006, 7(10): 2796-2805.
10.	Rnjak-Kovacina J, Wise SG, Li Z, et al. Tailoring the porosity and pore size of electrospun synthetic human elastin scaffolds for dermal tissue engineering. Biomaterials, 2011, 32(28): 6729-6736.
11.	Kwon IK, Kidoaki S, Matsuda T. Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: structural characteristics, mechanical properties and cell adhesion potential. Biomaterials, 2005, 26(18): 3929-3939.
12.	Thorvaldsson A, Stenhamre HS, Gatenholm P, et al. Electrospinning of highly porous scaffolds for cartilage regeneration. Biomacromolecules, 2008, 9(3): 1044-1049.
13.	Baker SC, Atkin N, Gunning PA, et al. Characterisation of electrospun polystyrene scaffolds for three-dimensional in vitro biological studies. Biomaterials, 2006, 27(16): 3136-3146.
14.	Matthews JA, Wnek GE, Simpson DG, et al. Electrospinning of collagen nanofibers. Biomacromolecules, 2002, 3(2): 232-238.
15.	Zhu X, Cui W, Li X, et al. Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. Biomacromolecules, 2008, 9(7): 1795-1801.
16.	McClure MJ, Wolfe PS, Simpson DG, et al. The use of air-flow impedance to control fiber deposition patterns during electrospinning. Biomaterials, 2012, 33(3): 771-779.
17.	Blakeney BA, Tambralli A, Anderson JM, et al. Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold. Biomaterials, 2011, 32(6): 1583-1590.
18.	Yokoyama Y, Hattori S, Yoshikawa C, et al. Novel wet electrospinning system for fabrication of spongiform nanofiber 3-dimensional fabric. Mater Lett, 2009, 63(9-10): 754-756.
19.	Ki CS, Park SY, Kim HJ, et al. Development of 3-d nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration. Biotechnol Lett, 2008, 30(3): 405-410.
20.	Zhang D, Chang J. Electrospinning of three-dimensional nanofibrous tubes with controllable architectures. Nano Lett, 2008, 8(10): 3283-3287.
21.	Vaquette C, Cooper-White JJ. Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration. Acta Biomaterialia, 2011, 7(6): 2544-2557.
22.	Lowery JL, Datta N, Rutledge GC. Effect of fiber diameter, pore size and seeding method on growth of human dermal fibroblasts in electrospun poly (epsilon-caprolactone) fibrous mats. Biomaterials, 2010, 31(3): 491-504.
23.	Baker BM, Gee AO, Metter RB, et al. The potential to improve cell infiltration in composite fiber-aligned electrospun scaffolds by the selective removal of sacrificial fibers. Biomaterials, 2008, 29(15): 2348-2358.
24.	Phipps MC, Clem WC, Grunda JM, et al. Increasing the pore sizes of bone-mimetic electrospun scaffolds comprised of polycaprolactone, collagen I and hydroxyapatite to enhance cell infiltration. Biomaterials, 2012, 33(2): 524-534.
25.	Skotak M, Ragusa J, Gonzalez D, et al. Improved cellular infiltration into nanofibrous electrospun cross-linked gelatin scaffolds templated with micrometer-sized polyethylene glycol fibers. Biomed Mater, 2011, 6(5): 055012.
26.	Annabi N, Nichol JW, Zhong X, et al. Controlling the porosity and microarchitecture of hydrogels for tissue engineering. Tissue Eng Part B Rev, 2010, 16(4): 371-383.
27.	Nam J, Huang Y, Agarwal S, et al. Improved cellular infiltration in electrospun fiber via engineered porosity. Tissue Eng, 2007, 13(9): 2249-2257.
28.	Simonet M, Schneider OD, Neuenschwander P, et al. Ultraporous 3D polymer meshes by low-temperature electrospinning: use of ice crystals as a removable void template. Polym Eng Sci, 2007, 47(12): 2020-2026.
29.	Leong MF, Rasheed MZ, Lim TC, et al. In vitro cell infiltration and in vivo cell infiltration and vascularization in a fibrous, highly porous poly (D, L-lactide) scaffold fabricated by cryogenic electrospinning technique. J Biomed Mater Res A, 2009, 91(1): 231-240.
30.	Lee JB, Jeong SI, Bae MS, et al. Highly porous electrospun nanofibers enhanced by ultrasonication for improved cellular infiltration. Tissue Eng Part A, 2011, 17(21-22): 2695-2702.
31.	Choi HW, Johnson JK, Nam J, et al. Structuring electrospun polycaprolactone nanofiber tissue scaffolds by femtosecond laser ablation. J Laser Appl, 2007, 19(4): 225-231.
32.	Sundararaghavan HG, Metter RB, Burdick JA. Electrospun fibrous scaffolds with multiscale and photopatterned porosity. Macromol Biosci, 2010, 10(3): 265-270.
33.	朱雷, 呂丹, 孫明林. 不同環境刺激對骨髓間充質干細胞成軟骨分化作用的研究進展. 中國矯形外科雜志, 2010, 18(19): 1618-1621.
34.	Mahmoudifar N, Doran PM. Chondrogenic differentiation of human adiposederived stem cells in polyglycolic acid mesh scaffolds under dynamic culture conditions. Biomaterials, 2010, 31(14): 3858-3867.
35.	Nerurkar NL, Sen S, Baker BM, et al. Dynamic culture enhances stem cell infiltration and modulates extracellular matrix production on aligned electrospun nanofibrous scaffolds. Acta Biomaterialia, 2011, 7(2): 485-491.
36.	Kenar H, Kose GT, Toner M, et al. A 3D aligned microfibrous myocardial tissue construct cultured under transient perfusion. Biomaterials, 2011, 32(23): 5320-5329.
37.	Stankus JJ, Guan J, Fujimoto K, et al. Microintegrating smooth muscle cells into a biodegradable, elastomeric fiber matrix. Biomaterials, 2006, 27(5): 735-744.
38.	Townsend-Nicholson A, Jayasinghe SN. Cell electrospinning: a unique biotechnique for encapsulating living organisms for generating active biological microthreads/scaffolds. Biomacromolecules, 2006, 7(12): 3364-3369.
39.	Yang XC, Shah JD, Wang HJ. Nanofiber enabled layer-by-layer approach toward three-dimensional tissue formation. Tissue Eng Part A, 2009, 15(4): 945-956.
40.	Park SH, Kim TG, Kim HC, et al. Development of dual scale scaffolds via direct polymer melt deposition and electrospinning for applications in tissue regeneration. Acta Biomater, 2008, 4(5): 1198-1207.

1. Agarwal S, Wendorff JH, Greiner A. Progress in the field of electrospinning for tissue engineering applications. Adv Mater, 2009, 21(32-33): 3343-3351.
2. 胡旭棟, 王光林. 靜電紡絲納米纖維支架在神經組織工程中的研究進展. 中國修復重建外科雜志, 2010, 24(9): 1133-1137.
3. 薛正翔, 李敏. 血管組織工程支架研究進展. 中國修復重建外科雜志, 2009, 23(9): 1134-1137.
4. Bhardwaj N, Kundu SC. Electrospinning: a fascinating fiber fabrication technique. Biotechnol Adv, 2010, 28(3): 325-347.
5. Rnjak-Kovacina J, Weiss AS. Increasing the pore size of electrospun scaffolds. Tissue Eng Part B Rev, 2011, 17(5): 365-372.
6. Liao S, Li B, Ma Z, et al. Biomimetic electrospun nanofibers for tissue regeneration. Biomed Mater, 2006, 1(3): R45-53.
7. Li M, Mondrinos MJ, Gandhi MR, et al. Electrospun protein fibers as matrices for tissue engineering. Biomaterials, 2005, 26(30): 5999-6008.
8. Eichhorn SJ, Sampson WW. Statistical geometry of pores and statistics of porous nanofibrous assemblies. J R Soc Interface, 2005, 2(4): 300-318.
9. Pham QP, Sharma U, Mikos AG. Electrospun poly (epsilon-caprolactone) microfiber and multilayer nanofiber/microfiber scaffolds: characterization of scaffolds and measurement of cellular infiltration. Biomacromolecules, 2006, 7(10): 2796-2805.
10. Rnjak-Kovacina J, Wise SG, Li Z, et al. Tailoring the porosity and pore size of electrospun synthetic human elastin scaffolds for dermal tissue engineering. Biomaterials, 2011, 32(28): 6729-6736.
11. Kwon IK, Kidoaki S, Matsuda T. Electrospun nano- to microfiber fabrics made of biodegradable copolyesters: structural characteristics, mechanical properties and cell adhesion potential. Biomaterials, 2005, 26(18): 3929-3939.
12. Thorvaldsson A, Stenhamre HS, Gatenholm P, et al. Electrospinning of highly porous scaffolds for cartilage regeneration. Biomacromolecules, 2008, 9(3): 1044-1049.
13. Baker SC, Atkin N, Gunning PA, et al. Characterisation of electrospun polystyrene scaffolds for three-dimensional in vitro biological studies. Biomaterials, 2006, 27(16): 3136-3146.
14. Matthews JA, Wnek GE, Simpson DG, et al. Electrospinning of collagen nanofibers. Biomacromolecules, 2002, 3(2): 232-238.
15. Zhu X, Cui W, Li X, et al. Electrospun fibrous mats with high porosity as potential scaffolds for skin tissue engineering. Biomacromolecules, 2008, 9(7): 1795-1801.
16. McClure MJ, Wolfe PS, Simpson DG, et al. The use of air-flow impedance to control fiber deposition patterns during electrospinning. Biomaterials, 2012, 33(3): 771-779.
17. Blakeney BA, Tambralli A, Anderson JM, et al. Cell infiltration and growth in a low density, uncompressed three-dimensional electrospun nanofibrous scaffold. Biomaterials, 2011, 32(6): 1583-1590.
18. Yokoyama Y, Hattori S, Yoshikawa C, et al. Novel wet electrospinning system for fabrication of spongiform nanofiber 3-dimensional fabric. Mater Lett, 2009, 63(9-10): 754-756.
19. Ki CS, Park SY, Kim HJ, et al. Development of 3-d nanofibrous fibroin scaffold with high porosity by electrospinning: implications for bone regeneration. Biotechnol Lett, 2008, 30(3): 405-410.
20. Zhang D, Chang J. Electrospinning of three-dimensional nanofibrous tubes with controllable architectures. Nano Lett, 2008, 8(10): 3283-3287.
21. Vaquette C, Cooper-White JJ. Increasing electrospun scaffold pore size with tailored collectors for improved cell penetration. Acta Biomaterialia, 2011, 7(6): 2544-2557.
22. Lowery JL, Datta N, Rutledge GC. Effect of fiber diameter, pore size and seeding method on growth of human dermal fibroblasts in electrospun poly (epsilon-caprolactone) fibrous mats. Biomaterials, 2010, 31(3): 491-504.
23. Baker BM, Gee AO, Metter RB, et al. The potential to improve cell infiltration in composite fiber-aligned electrospun scaffolds by the selective removal of sacrificial fibers. Biomaterials, 2008, 29(15): 2348-2358.
24. Phipps MC, Clem WC, Grunda JM, et al. Increasing the pore sizes of bone-mimetic electrospun scaffolds comprised of polycaprolactone, collagen I and hydroxyapatite to enhance cell infiltration. Biomaterials, 2012, 33(2): 524-534.
25. Skotak M, Ragusa J, Gonzalez D, et al. Improved cellular infiltration into nanofibrous electrospun cross-linked gelatin scaffolds templated with micrometer-sized polyethylene glycol fibers. Biomed Mater, 2011, 6(5): 055012.
26. Annabi N, Nichol JW, Zhong X, et al. Controlling the porosity and microarchitecture of hydrogels for tissue engineering. Tissue Eng Part B Rev, 2010, 16(4): 371-383.
27. Nam J, Huang Y, Agarwal S, et al. Improved cellular infiltration in electrospun fiber via engineered porosity. Tissue Eng, 2007, 13(9): 2249-2257.
28. Simonet M, Schneider OD, Neuenschwander P, et al. Ultraporous 3D polymer meshes by low-temperature electrospinning: use of ice crystals as a removable void template. Polym Eng Sci, 2007, 47(12): 2020-2026.
29. Leong MF, Rasheed MZ, Lim TC, et al. In vitro cell infiltration and in vivo cell infiltration and vascularization in a fibrous, highly porous poly (D, L-lactide) scaffold fabricated by cryogenic electrospinning technique. J Biomed Mater Res A, 2009, 91(1): 231-240.
30. Lee JB, Jeong SI, Bae MS, et al. Highly porous electrospun nanofibers enhanced by ultrasonication for improved cellular infiltration. Tissue Eng Part A, 2011, 17(21-22): 2695-2702.
31. Choi HW, Johnson JK, Nam J, et al. Structuring electrospun polycaprolactone nanofiber tissue scaffolds by femtosecond laser ablation. J Laser Appl, 2007, 19(4): 225-231.
32. Sundararaghavan HG, Metter RB, Burdick JA. Electrospun fibrous scaffolds with multiscale and photopatterned porosity. Macromol Biosci, 2010, 10(3): 265-270.
33. 朱雷, 呂丹, 孫明林. 不同環境刺激對骨髓間充質干細胞成軟骨分化作用的研究進展. 中國矯形外科雜志, 2010, 18(19): 1618-1621.
34. Mahmoudifar N, Doran PM. Chondrogenic differentiation of human adiposederived stem cells in polyglycolic acid mesh scaffolds under dynamic culture conditions. Biomaterials, 2010, 31(14): 3858-3867.
35. Nerurkar NL, Sen S, Baker BM, et al. Dynamic culture enhances stem cell infiltration and modulates extracellular matrix production on aligned electrospun nanofibrous scaffolds. Acta Biomaterialia, 2011, 7(2): 485-491.
36. Kenar H, Kose GT, Toner M, et al. A 3D aligned microfibrous myocardial tissue construct cultured under transient perfusion. Biomaterials, 2011, 32(23): 5320-5329.
37. Stankus JJ, Guan J, Fujimoto K, et al. Microintegrating smooth muscle cells into a biodegradable, elastomeric fiber matrix. Biomaterials, 2006, 27(5): 735-744.
38. Townsend-Nicholson A, Jayasinghe SN. Cell electrospinning: a unique biotechnique for encapsulating living organisms for generating active biological microthreads/scaffolds. Biomacromolecules, 2006, 7(12): 3364-3369.
39. Yang XC, Shah JD, Wang HJ. Nanofiber enabled layer-by-layer approach toward three-dimensional tissue formation. Tissue Eng Part A, 2009, 15(4): 945-956.
40. Park SH, Kim TG, Kim HC, et al. Development of dual scale scaffolds via direct polymer melt deposition and electrospinning for applications in tissue regeneration. Acta Biomater, 2008, 4(5): 1198-1207.

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促進靜電紡絲支架中細胞滲透生長的研究進展

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《中國修復重建外科雜志》

促進靜電紡絲支架中細胞滲透生長的研究進展

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