生物反應器生物物理因素在軟骨組織工程中的研究進展_《中國修復重建外科雜志》

作者：

葉鋼 , 章方彪 , 史宏燦

揚州大學醫學院胸外科（江蘇揚州，225001）;

關鍵詞：

軟骨組織工程生物反應器氧濃度靜水壓壓縮力剪切力

DOI：

10.7507/1002-1892.20130178

視頻：

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摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的綜述生物反應器生物物理因素在軟骨組織工程領域的研究進展。方法查閱生物反應器生物物理因素在軟骨組織工程領域中相關文獻，并進行總結分析。結果生物反應器中氧濃度、靜水壓、壓縮力和剪切力四大生物物理因素最適參數均無統一標準，其中靜水壓和剪切力尚存在爭議，制約了生物反應器的應用。結論軟骨組織工程生物反應器中生物物理因素仍需深入研究。

引用本文： 葉鋼,章方彪,史宏燦. 生物反應器生物物理因素在軟骨組織工程中的研究進展. 中國修復重建外科雜志, 2013, 27(7): 810-813. doi: 10.7507/1002-1892.20130178 復制

1.	Oragui E, Nannaparaju M, Khan WS. The role of bioreactors in tissue engineering for musculoskeletal applications. Open Orthop J, 2011, 5 Suppl 2: 267-270.
2.	Mabvuure N, Hindocha S, Khan WS. The role of bioreactors in cartilage tissue engineering. Curr Stem Cell Res Ther, 2012, 7(4): 287-292.
3.	Govoni M, Lotti F, Biagiotti L, et al. An innovative stand-alone bioreactor for the highly reproducible transfer of cyclic mechanical stretch to stem cells cultured in a 3D scaffold. J Tissue Eng Regen Med, 2012. [Epub ahead of print].
4.	Hoening E, Winkler T, Mielke G, et al. High amplitude direct compressive strain enhances mechanical properties of scaffold-free tissue engineered cartilage. Tissue Eng Part A, 2011, 17(9-10): 1401-1411.
5.	Cinbiz MN, Ti?li RS, Be?karde? IG, et al. Computational fluid dynamies modeling of momentum transport in rotating wall perfused bioreactor for cartilage tissue engineering. J Biotechnol, 2010, 150(3): 389-395.
6.	Malda J, Martens DE, Tramper J, et al. Cartilage tissue engineering: controversy in the effect of oxygen. Crit Rev Biotechnol, 2003, 23(3): 175-194.
7.	Wernike E, Li Z, Alini M, et al. Effect of reduced oxygen tension and long-term mechanical stimulation on chondrocyte-polymer constructs. Cell Tissue Res, 2008, 331(2): 473-483.
8.	Das RH, van Osch GJ, Kreukniet M, et al. Effects of individual control of pH and hypoxia in chondrocyte culture. J Orthop Res, 2010, 28(4): 537-545.
9.	Lovett M, Rockwood D, Baryshyan A, et al. Simple modular bioreactors for tissue engineering: a system for characterization of oxygen gradients, human mesenchymal stemcell differentiation and prevascularization. Tissue Eng Part C Methods, 2010, 16(6): 1565-1573.
10.	Meyer EG, Buckley CT, Thorpe SD, et al. Low oxygen tension is a more potent promoter of chondrogenic differentiation than dynamic compression. J Biomech, 2010, 43(13): 2516-2523.
11.	Schrobback K, Klein TJ, Crawford R, et al. Effects of oxygen and culture system on in vitro propagation and redifferentiation of osteoarthritic human articular chondrocytes. Cell Tissue Res, 2012, 347(3): 649-663.
12.	Terkhorn SP, Bohensky J, Shapiro IM, et al. Expression of HIF prolyl hydroxylase isozymes in growth plate chondrocytes: relationship between maturation and apoptotic sensitivity. J Cell Physiol, 2007, 210(1): 257-265.
13.	Pörtner R, Goepfert C, Wiegandt K, et al. Technical strategies to improve tissue engineering of cartilage-carrier-constructs. Adv Biochem Eng Biotechnol, 2009, 112: 145-181.
14.	Schulz RM, Bader A. Cartilage tissue engineering and bioreactor systems for the cultivation and stimulation of chondrocytes. Eur Biophys J, 2007, 36(4-5): 539-568.
15.	Ogawa R, Mizuno S, Murphy GF, et al. The effect of hydrostatic pressure on three-dimensional chondroinduction of human adipose-derived stem cells. Tissue Eng Part A, 2009, 15(10): 2937-2945.
16.	Elder BD, Athanasiou KA. Hydrostatic pressure in articular cartilage tissue engineering: from chondrocytes to tissue regeneration. Tissue Eng Part B Rev, 2009, 15(1): 43-53.
17.	Jeong JY, Park SH, Shin JW, et al. Effects of intermittent hydrostatic ressure magnitude on the chondrogenesis of MSCs without biochemical agents under 3D co-culture. J Mater Sci Mater Med, 2012, 23(11): 2773-2781.
18.	Takahashi I, Nuckolls GH, Takahashi K, et al. Compressive force promotes sox9, type II collagen and aggrecan and inhibits IL-1beta expression resulting in chondrogenensis in mouse embryonic limb bud mesenchymal cells. J Cell Sci, 1998, 111(Pt 14): 2067-2076.
19.	Nugent GE, Schmidt TA, Schumacher BL, et al. Static and dynamic compression regulate cartilage metabolism of PRoteoGlycan 4 (PRG4). Biorheology, 2006, 43(3-4): 191-200.
20.	Li KW, Williamson AK, Wang AS, et al. Growth responses of cartilage to static and dynamic compression. Clin Orthop Relat Res, 2001, (391 Suppl): S34-48.
21.	Park S, Hung CT, Ateshian GA. Mechanical response of bovine articular cartilage under dynamic unconfined compression loading at physiological stress levels. Osteoarthritis Cartilage, 2004, 12(1): 65-73.
22.	Haugh MG, Meyer EG, Thorpe SD, et al. Temporal and spatial changes in cartilage-matrix-specific gene expression in mesenchymal stem cells in response to dynamic compression. Tissue Eng Part A, 2011, 17(23-24): 3085-3093.
23.	Schätti O, Grad S, Goldhahn J, et al. A combination of shear and dynamic compression leads to mechanically induced chondrogenesis of human mesenchymal stem cells. Eur Cell Mater, 2011, 22: 214-225.
24.	Liu C, Abedian R, Meister R, et al. Influence of perfusion and compression on the proliferation and differentiation of bone mesenchymal stromal cells seeded on polyurethane scaffolds. Biomaterials, 2012, 33(4): 1052-1064.
25.	Huang AH, Farrell MJ, Mauck RL. Mechanics and mechanobiology of mesenchymal stem cell-based engineered cartilage. J Biomech, 2010, 43(1): 128-136.
26.	Richardson SM, Hoyland JA, Mobasheri R, et al. Mesenchymal stem cells in regenerative medicine: Opportunities and challenges for articu-lar cartilage and intervertebral disc tissue engineering. J Cell Physiol, 2010, 222(1): 23-32.
27.	Hoenig E, Winkler T, Mielke G, et al. High amplitude direct compressive strain enhances mechanical properties of scaffold-free tissue-engineered cartilage. Tissue Eng Part A, 2011, 17(9-10): 1401-1411.
28.	孫明林, 朱雷, 呂丹, 等. 不同參數滾壓模式對兔BMSCs向軟骨細胞分化促進作用的研究. 中國修復重建外科雜志, 2013, 27(1): 89-94.
29.	Huang AH, Farrell MJ, Kim M, et al. Long-term dynamic loading improves the mechanical properties of chondrogenic mesenchymal stem cell-laden hydrogel. Eur Cell Mater, 2010, 19: 72-85.
30.	Thorpe SD, Buckley CT, Vinardell T, et al. Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells. Biochem Biophys Res Commun, 2008, 377(2): 458-462.
31.	Zhu F, Wang P, Lee NH, et al. Prolonged application of high fluid shear to chondrocytes recapitulates gene expression profiles associated with osteoarthritis. PLoS One, 2010, 5(12): e15174.
32.	Wang P, Zhu F, Tong Z, et al. Response of chondrocytes to shear stress: antagonistic effects of the binding partners Toll-like receptor 4 and caveolin-1. FASEB J, 2011, 25(10): 3401-3415.
33.	Wang P, Zhu F, Lee NH, et al. Shear-induced interleukin-6 synthesis in chondrocyte: roles of E prostanoid (EP) 2 and EP3 in cAMP/proteinkinase A-and PI3-K/Akt-dependent NF-kappa B activation. J Biol Chem, 2010, 285(32): 24793-24804.
34.	謝甲琦, 魏旭峰, 康小軍, 等. 剪應力誘導骨髓間充質干細胞向內皮細胞分化. 第四軍醫大學學報, 2009, 30(7): 588-590.
35.	Hambor JE. Bioreactor design and bioprocess controls for industrialized cell processing. BioProcess Int, 2012, 10(6): 22-33.

1. Oragui E, Nannaparaju M, Khan WS. The role of bioreactors in tissue engineering for musculoskeletal applications. Open Orthop J, 2011, 5 Suppl 2: 267-270.
2. Mabvuure N, Hindocha S, Khan WS. The role of bioreactors in cartilage tissue engineering. Curr Stem Cell Res Ther, 2012, 7(4): 287-292.
3. Govoni M, Lotti F, Biagiotti L, et al. An innovative stand-alone bioreactor for the highly reproducible transfer of cyclic mechanical stretch to stem cells cultured in a 3D scaffold. J Tissue Eng Regen Med, 2012. [Epub ahead of print].
4. Hoening E, Winkler T, Mielke G, et al. High amplitude direct compressive strain enhances mechanical properties of scaffold-free tissue engineered cartilage. Tissue Eng Part A, 2011, 17(9-10): 1401-1411.
5. Cinbiz MN, Ti?li RS, Be?karde? IG, et al. Computational fluid dynamies modeling of momentum transport in rotating wall perfused bioreactor for cartilage tissue engineering. J Biotechnol, 2010, 150(3): 389-395.
6. Malda J, Martens DE, Tramper J, et al. Cartilage tissue engineering: controversy in the effect of oxygen. Crit Rev Biotechnol, 2003, 23(3): 175-194.
7. Wernike E, Li Z, Alini M, et al. Effect of reduced oxygen tension and long-term mechanical stimulation on chondrocyte-polymer constructs. Cell Tissue Res, 2008, 331(2): 473-483.
8. Das RH, van Osch GJ, Kreukniet M, et al. Effects of individual control of pH and hypoxia in chondrocyte culture. J Orthop Res, 2010, 28(4): 537-545.
9. Lovett M, Rockwood D, Baryshyan A, et al. Simple modular bioreactors for tissue engineering: a system for characterization of oxygen gradients, human mesenchymal stemcell differentiation and prevascularization. Tissue Eng Part C Methods, 2010, 16(6): 1565-1573.
10. Meyer EG, Buckley CT, Thorpe SD, et al. Low oxygen tension is a more potent promoter of chondrogenic differentiation than dynamic compression. J Biomech, 2010, 43(13): 2516-2523.
11. Schrobback K, Klein TJ, Crawford R, et al. Effects of oxygen and culture system on in vitro propagation and redifferentiation of osteoarthritic human articular chondrocytes. Cell Tissue Res, 2012, 347(3): 649-663.
12. Terkhorn SP, Bohensky J, Shapiro IM, et al. Expression of HIF prolyl hydroxylase isozymes in growth plate chondrocytes: relationship between maturation and apoptotic sensitivity. J Cell Physiol, 2007, 210(1): 257-265.
13. Pörtner R, Goepfert C, Wiegandt K, et al. Technical strategies to improve tissue engineering of cartilage-carrier-constructs. Adv Biochem Eng Biotechnol, 2009, 112: 145-181.
14. Schulz RM, Bader A. Cartilage tissue engineering and bioreactor systems for the cultivation and stimulation of chondrocytes. Eur Biophys J, 2007, 36(4-5): 539-568.
15. Ogawa R, Mizuno S, Murphy GF, et al. The effect of hydrostatic pressure on three-dimensional chondroinduction of human adipose-derived stem cells. Tissue Eng Part A, 2009, 15(10): 2937-2945.
16. Elder BD, Athanasiou KA. Hydrostatic pressure in articular cartilage tissue engineering: from chondrocytes to tissue regeneration. Tissue Eng Part B Rev, 2009, 15(1): 43-53.
17. Jeong JY, Park SH, Shin JW, et al. Effects of intermittent hydrostatic ressure magnitude on the chondrogenesis of MSCs without biochemical agents under 3D co-culture. J Mater Sci Mater Med, 2012, 23(11): 2773-2781.
18. Takahashi I, Nuckolls GH, Takahashi K, et al. Compressive force promotes sox9, type II collagen and aggrecan and inhibits IL-1beta expression resulting in chondrogenensis in mouse embryonic limb bud mesenchymal cells. J Cell Sci, 1998, 111(Pt 14): 2067-2076.
19. Nugent GE, Schmidt TA, Schumacher BL, et al. Static and dynamic compression regulate cartilage metabolism of PRoteoGlycan 4 (PRG4). Biorheology, 2006, 43(3-4): 191-200.
20. Li KW, Williamson AK, Wang AS, et al. Growth responses of cartilage to static and dynamic compression. Clin Orthop Relat Res, 2001, (391 Suppl): S34-48.
21. Park S, Hung CT, Ateshian GA. Mechanical response of bovine articular cartilage under dynamic unconfined compression loading at physiological stress levels. Osteoarthritis Cartilage, 2004, 12(1): 65-73.
22. Haugh MG, Meyer EG, Thorpe SD, et al. Temporal and spatial changes in cartilage-matrix-specific gene expression in mesenchymal stem cells in response to dynamic compression. Tissue Eng Part A, 2011, 17(23-24): 3085-3093.
23. Schätti O, Grad S, Goldhahn J, et al. A combination of shear and dynamic compression leads to mechanically induced chondrogenesis of human mesenchymal stem cells. Eur Cell Mater, 2011, 22: 214-225.
24. Liu C, Abedian R, Meister R, et al. Influence of perfusion and compression on the proliferation and differentiation of bone mesenchymal stromal cells seeded on polyurethane scaffolds. Biomaterials, 2012, 33(4): 1052-1064.
25. Huang AH, Farrell MJ, Mauck RL. Mechanics and mechanobiology of mesenchymal stem cell-based engineered cartilage. J Biomech, 2010, 43(1): 128-136.
26. Richardson SM, Hoyland JA, Mobasheri R, et al. Mesenchymal stem cells in regenerative medicine: Opportunities and challenges for articu-lar cartilage and intervertebral disc tissue engineering. J Cell Physiol, 2010, 222(1): 23-32.
27. Hoenig E, Winkler T, Mielke G, et al. High amplitude direct compressive strain enhances mechanical properties of scaffold-free tissue-engineered cartilage. Tissue Eng Part A, 2011, 17(9-10): 1401-1411.
28. 孫明林, 朱雷, 呂丹, 等. 不同參數滾壓模式對兔BMSCs向軟骨細胞分化促進作用的研究. 中國修復重建外科雜志, 2013, 27(1): 89-94.
29. Huang AH, Farrell MJ, Kim M, et al. Long-term dynamic loading improves the mechanical properties of chondrogenic mesenchymal stem cell-laden hydrogel. Eur Cell Mater, 2010, 19: 72-85.
30. Thorpe SD, Buckley CT, Vinardell T, et al. Dynamic compression can inhibit chondrogenesis of mesenchymal stem cells. Biochem Biophys Res Commun, 2008, 377(2): 458-462.
31. Zhu F, Wang P, Lee NH, et al. Prolonged application of high fluid shear to chondrocytes recapitulates gene expression profiles associated with osteoarthritis. PLoS One, 2010, 5(12): e15174.
32. Wang P, Zhu F, Tong Z, et al. Response of chondrocytes to shear stress: antagonistic effects of the binding partners Toll-like receptor 4 and caveolin-1. FASEB J, 2011, 25(10): 3401-3415.
33. Wang P, Zhu F, Lee NH, et al. Shear-induced interleukin-6 synthesis in chondrocyte: roles of E prostanoid (EP) 2 and EP3 in cAMP/proteinkinase A-and PI3-K/Akt-dependent NF-kappa B activation. J Biol Chem, 2010, 285(32): 24793-24804.
34. 謝甲琦, 魏旭峰, 康小軍, 等. 剪應力誘導骨髓間充質干細胞向內皮細胞分化. 第四軍醫大學學報, 2009, 30(7): 588-590.
35. Hambor JE. Bioreactor design and bioprocess controls for industrialized cell processing. BioProcess Int, 2012, 10(6): 22-33.

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生物反應器生物物理因素在軟骨組織工程中的研究進展

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《中國修復重建外科雜志》

生物反應器生物物理因素在軟骨組織工程中的研究進展

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