caspase-3在納米磁藥物靶向治療膽管移植瘤組織中的表達_《中國普外基礎與臨床雜志》

作者：

李宏 , 鄭建偉 , 唐滔 , 鄒聲泉

華中科技大學同濟醫學院附屬同濟醫院普通外科（武漢430030）;

關鍵詞：

納米磁藥物膽管癌半胱氨酸天冬氨酸特異性蛋白酶-3

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目的　探討納米磁藥物靶向治療膽管移植瘤組織中凋亡相關基因caspase-3的表達。方法　建立荷人膽管癌裸鼠模型，隨機分為空白對照組、納米磁藥物組(藥物濃度為250 mg/kg)、納米磁藥物靶向A組(藥物濃度為150 mg/kg)和納米磁藥物靶向B組(藥物濃度為250 mg/kg)；取治療后第21 d的各組腫瘤組織用免疫組化及RT-PCR法測量其中caspase-3的蛋白及mRNA表達量并進行分析。結果　納米磁藥物靶向B組中caspase-3的表達量最高,其次依次為納米磁藥物靶向A組、納米磁藥物組，空白對照組幾乎沒有caspase-3蛋白及mRNA的表達，且各組間差異均有統計學意義（P＜0.05)。結論　磁靶向治療腫瘤后能引起更多凋亡相關基因caspase-3表達，并且隨納米磁藥物劑量增加，caspase-3表達增多。

引用本文： 李宏,鄭建偉,唐滔,鄒聲泉. caspase-3在納米磁藥物靶向治療膽管移植瘤組織中的表達. 中國普外基礎與臨床雜志, 2006, 13(6): 685-688. doi: 復制

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2. 　Aoyagi T, Okano T. Targeting of anticancer drug using intelligent polymers [J]. Nippon Rinsho, 1998; 56(3)∶644.
3. 　Senyei AE, Widder KJ. Drug targeting: magnetically responsive albumin microspheres——a review of the system to date [J]. Gynecol Oncol, 1981; 12(1)∶1.
4. 　呂　軍, 楊連粵, 楊建青. 5-氟尿嘧啶誘導肝癌細胞凋亡過程中 caspase-3活性變化的研究 [J]. 中華實驗外科雜志， 2000； 17（5）∶401.
5. 　Widder KJ, Marino PA, Morris RM, et al. Selective targeting of magnetic albumin microspheres to the Yoshida sarcoma: ultrastructural evaluation of microsphere disposition [J]. Eur J Cancer Clin Oncol, 1983; 19(1)∶141.
6. 　Zou W, Zeng J, Zhou M, et al. Involvement of caspase-3 and p38 mitogen-activated protein kinase in cobalt chloride-induced apoptosis in PC12 cells [J]. J Neurosci Res, 2002; 67(6)∶837.
7. 　Fernandes-Alnemri T, Litwack G, Alnemri ES. CPP32, a novel human apoptotic protein with homology to Caenorhabditis elegans cell death protein Ced-3 and mammalian interleukin-1 beta-converting enzyme [J].J Biol Chem, 1994; 269(49)∶30761.
8. 　Cosulich SC, Savory PJ, Clarke PR. Bcl-2 regulates amplification of caspase activation by cytochrome c[J]. Curr Biol, 1999; 9(3)∶147.
9. 　Yeh JH, Hsu SC, Han SH, et al. Mitogen-activated protein kinase kinase antagonized fas-associated death domain protein-mediated apoptosis by induced FLICE-inhibitory protein expression [J]. J Exp Med, 1998; 188(10)∶1795.

《中國普外基礎與臨床雜志》

caspase-3在納米磁藥物靶向治療膽管移植瘤組織中的表達

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《中國普外基礎與臨床雜志》

caspase-3在納米磁藥物靶向治療膽管移植瘤組織中的表達

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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