過氧化氫對A549細胞轉化生長因子β1和Smad3表達的影響_《中國呼吸與危重監護雜志》

作者：

楊冬梅 ¹ , 丁宇 ¹ , 應斌武 ^1,2 , 蔣飛 ¹ , 徐丹 ¹ , 美朗曲措 ¹ ,  范紅 ^1,2

1 四川大學華西醫院呼吸內科（四川成都 610041）;;
2 四川大學華西醫院實驗醫學科;

關鍵詞：

過氧化氫轉化生長因子 β1 Smad3

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目的研究過氧化氫對 A549 細胞表達轉化生長因子 β1 （ TGF-β1 ）和 Smad3 磷酸化的影響。方法用過氧化氫建立體外A549細胞損傷模型，MTT法檢測過氧化氫對A549細胞增殖活性的影響，免疫印跡法（Western Blotting）觀察過氧化氫作用不同時間后A549細胞TGF-β1和Smad 3磷酸化的表達。結果過氧化氫明顯抑制A549細胞的增殖，濃度為1.0 mmol/L時，其對A549細胞增殖的抑制率為46.34%；A549細胞在過氧化氫（1.0 mmol/L）的刺激下，TGF-β1和Smad3磷酸化的表達量逐漸升高，24 h時達到高峰, 48 h下降。結論氧化損傷早期，A549細胞表達TGF-β1和Smad3磷酸化增加,這可能與肺損傷后依賴Smad3的組織修復有關。

引用本文： 楊冬梅 ,丁宇,應斌武,蔣飛,徐丹,美朗曲措,范紅. 過氧化氫對A549細胞轉化生長因子β1和Smad3表達的影響. 中國呼吸與危重監護雜志, 2008, 08(1): 43-46. doi: 復制

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2. Chabot F, Mitchell JA, Gutteridge JM, et al. Reactive oxygen species in acute lung injury. Eur Respir J, 1998,11:745-757.
3. Waxman AB, Mahboubi K, Knickelbein RG,et al. Interleukin-11 and interleukin-6 protect cultured human endothelial cells from H2O2-induced cell death. Am J Respir Cell Mol Biol, 2003,29:513-522.
4. Bellingan GJ.The pulmonary physician in critical care 6: The pathogenesis of ALI/ARDS.Thorax,2002,57:540-546.
5. 陳謙.二喹啉甲酸（BCA）檢測法.見：汪家政，范明，主編.蛋白質技術手冊．北京：科學出版社，2001，51-54．.
6. Fehrenbach H. Alveolar epithelial type II cell: defender of the alveolus revisited. Respir Res,2001,2:33-46.
7. 張靜，吳瑜.N-乙酰-L-半胱氨酸對H2O2引起的血管內皮細胞損傷的拮抗效應.中國呼吸與危重監護雜志，2003，2：91-94.
8. Derynck R, Zhang YE. Smad-dependent and Smad-independent pathways in TGF-beta family signalling. Nature,2003,425:577-584.
9. Ebner R, Chen RH, Lawler S, et al . Determination of type I receptor specificity by the type II receptors for TGF-beta or activin.Science,1993,262:900-902.
10. Verrecchia F, Chu ML, Mauviel A.Identification of novel TGF-beta /Smad gene targets in dermal fibroblasts using a combined cDNA microarray/promoter transactivation approach.J Biol Chem,2001,276:17058-17062.
11. Muthukrishnan L, Warder E, McNeil PL.Basic fibroblast growth factor is efficiently released from a cytolsolic storage site through plasma membrane disruptions of endothelial cells. J Cell Physiol,1991,148:1-16.

《中國呼吸與危重監護雜志》

過氧化氫對A549細胞轉化生長因子β1和Smad3表達的影響

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《中國呼吸與危重監護雜志》

過氧化氫對A549細胞轉化生長因子β1和Smad3表達的影響

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