呼吸道合胞病毒感染的大鼠氣道上皮細胞對樹突狀細胞的影響_《中國呼吸與危重監護雜志》

作者：

喬建甌 ¹ , 金先橋 ²

1 上海交通大學附屬第九人民醫院呼吸科( 上海 200010) ;;
2 上海交通大學附屬第一人民醫院呼吸科( 上海 200080)通訊作者: 金先橋, E-mail: jinxianqiao@ hotmail. com;

關鍵詞：

呼吸道合胞病毒氣道上皮細胞樹突狀細胞哮喘 Th2 細胞

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目的探索呼吸道合胞病毒( RSV) 感染的氣道上皮細胞對大鼠髓系樹突狀細胞( mDCs) 激活和功能的影響, 為研究RSV 誘發哮喘的機制提供依據。方法大鼠氣道上皮細胞株進
行培養。在對RSV 進行擴增和滴度測定后, 以不同作用時間、不同滴度的RSV 感染大鼠氣道上皮細胞。同時分離、培養擴增大鼠mDCs, 采用transwell 細胞共培養系統將大鼠氣道上皮細胞和大鼠mDCs共培養。其mDCs 通過RT-PCR 和流式細胞術檢測成熟標志物和Th2 細胞因子的表達、同種異體混合淋巴細胞反應( MLR) 。結果大鼠mDCs 與RSV 感染的大鼠氣道上皮細胞共培養后出現功能性成熟, 包括大鼠mDCs 表面共刺激分子MHCⅡ 和CD86 的表達增多, OX40L 和TARC 的mRNAs 表達增高, 大鼠mDCs 刺激T 細胞增殖的能力增強。結論 RSV 感染大鼠氣道上皮細胞能夠促使大鼠mDCs 進一步成熟并且可能具有支持Th2 細胞分化和局部聚集的能力, 可能是RSV 誘發哮喘的一種機制。

引用本文： 喬建甌,金先橋. 呼吸道合胞病毒感染的大鼠氣道上皮細胞對樹突狀細胞的影響. 中國呼吸與危重監護雜志, 2010, 9(6): 602-605. doi: 復制

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10.	Tsuyuki S, Tsuyuki J, Einsle K, et al. Costimulation through B7-2( CD86) is required for the induction of a lung mucosal T helper cell 2 ( TH2) immune response and altered airway responsiveness. J Exp Med, 1997, 185 : 1671 -1679 .
11.	Imai T, Yoshida T, Baba M, et al. Molecular cloning of a novel T celldirected CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector. J Biol Chem,1996,271:21514-21521.
12.	Bonecchi R, Bianchi G, Bordignon PP, et al. Differential express, on of chemokine receptors and chamotactic responsiveness of type1 Thelper cells( Thls) and Th2s. J Exp Med, 1998 , 187: 129-134 .
13.	Leung TF, Wong CK. Chan IH, et al. Plasma cocentration of thymus and activatlon-regulated chemokine is elevated in childhood asthma.Jallergy Clin lmmunol, 2002, 110: 404 -409.

1. 李明才, 何韶衡. 病毒感染在誘發和加重哮喘中的作用. 中國呼吸與危重監護雜志, 2003, 2: 189-192.
2. Becker S, Reed W, Henderson FW, et al. RSV infection of human airway epithelial cells causes production of the beta-chemokine RANTES. Am J Physiol, 1997 , 272: L512-L520 .
3. Zhang Y, Luxon BA, Casola A, et al. Expression of respiratory syncytial virus-induced chemokine gene networks in lower airway epithelial cells revealed by cDNA microarrays. J Virol,2001, 75:9044-9058.
4. 毛光宇, 楊炯, 陳宏斌, 等. 過敏性哮喘患者樹突狀細胞對原始T細胞活化的影響. 中國呼吸與危重監護雜志, 2005,4: 115-118.
5. Mohapatra SS, Boyapalle S. Epidemiologic, experimental, and clinical links between respiratory syncytial virus infection and asthma. Clin Microbiol Rev, 2008, 21: 495-504.
6. Lambrecht BN, De Veerman M, Coyle AJ, et al. Myeloid dendritic cells induce Th2 responsesto inhaled antigen, leading to eosinophilic airway inflammation. J Clin Invest, 2000 , 106: 551-559.
7. McAdam AJ, Schweitzer AN, Sharpe AH. The role of B7 costimulation in activation and differentiation of CD41 and CD81 T cells. Immunol Rev, 1998 , 165: 231-247 .
8. Keane-Myers AM, Gause WC, Finkelman FD, et al. Development of murine allergic asthma is dependent upon B7-2 costimulation. J Immunol, 1998, 160: 1036-1043 .
9. Lordan JL, Davies DE, Wilson SJ, et al. The role of CD28-B7 costimulation in allergen-induced cytokine release by bronchial mucosa from patients with moderately severe asthma. J Allergy Clin Immunol, 2001, 108: 976-981 .
10. Tsuyuki S, Tsuyuki J, Einsle K, et al. Costimulation through B7-2( CD86) is required for the induction of a lung mucosal T helper cell 2 ( TH2) immune response and altered airway responsiveness. J Exp Med, 1997, 185 : 1671 -1679 .
11. Imai T, Yoshida T, Baba M, et al. Molecular cloning of a novel T celldirected CC chemokine expressed in thymus by signal sequence trap using Epstein-Barr virus vector. J Biol Chem,1996,271:21514-21521.
12. Bonecchi R, Bianchi G, Bordignon PP, et al. Differential express, on of chemokine receptors and chamotactic responsiveness of type1 Thelper cells( Thls) and Th2s. J Exp Med, 1998 , 187: 129-134 .
13. Leung TF, Wong CK. Chan IH, et al. Plasma cocentration of thymus and activatlon-regulated chemokine is elevated in childhood asthma.Jallergy Clin lmmunol, 2002, 110: 404 -409.

《中國呼吸與危重監護雜志》

呼吸道合胞病毒感染的大鼠氣道上皮細胞對樹突狀細胞的影響

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《中國呼吸與危重監護雜志》

呼吸道合胞病毒感染的大鼠氣道上皮細胞對樹突狀細胞的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料