小鼠脛骨中段1/3不同缺損直徑單層骨皮質缺損模型比較研究_《中國修復重建外科雜志》

作者：

李福兵 ¹ , 徐永清 ¹ , 潘興華 ² , 李霞 ¹ , 劉華 ¹ , 楊杜明 ¹ , 李軍 ¹ , 沙勇 ¹ , 石健 ¹ , 趙萬秋 ¹

成都軍區昆明總醫院（昆明，650032）1 骨科全軍創傷骨科研究所，2 干細胞與組織器官工程中心;

關鍵詞：

脛骨骨缺損動物模型小鼠

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目的比較研究不同缺損直徑對小鼠脛骨中段1/3單層骨皮質缺損模型愈合的影響，為組織工程材料研究、骨缺損修復及其分子機制研究和骨缺損基因治療研究等提供動物模型。方法取8周齡雄性C57BL/6J小鼠10只，體重（20 ± 2）g，隨機分為A、B兩組，每組5只。利用牙科磨鉆分別制備直徑為0.8 mm（A組）和1.0 mm（B組）小鼠脛骨中段1/3單層骨皮質缺損模型。于造模后7、21、28 d攝鉬靶X線片觀察缺損修復情況；28 d對骨缺損修復行Micro CT掃描及骨組織三維成像；28 d取材行HE染色觀察。結果B組5只小鼠造模7 d內均發生二次骨折，A組無骨折發生。X線片、Micro CT和HE染色均顯示A組脛骨單層骨皮質缺損可在28 d達骨性愈合。Micro CT定量分析骨小梁示，A組骨小梁數目、骨小梁密度、骨體積顯著高于B組，骨質密度顯著低于B組，差異均有統計學意義（P lt; 0.05）；兩組骨小梁分離度、骨小梁厚度差異無統計學意義（P gt; 0.05）。結論小鼠脛骨中段1/3單層直徑0.8 mm骨皮質缺損模型是研究脛骨缺損無外固定缺損修復機制和骨替代植入材料的理想動物模型。

引用本文： 李福兵,徐永清,潘興華,李霞,劉華,楊杜明,李軍,沙勇,石健,趙萬秋. 小鼠脛骨中段1/3不同缺損直徑單層骨皮質缺損模型比較研究. 中國修復重建外科雜志, 2012, 26(10): 1218-1222. doi: 復制

1.	Masquelet AC, Beque T. The concept of induced membrane for reconstruction of long bone defects. Orthop Clin North Am, 2010, 41(1): 27-37.
2.	Wiese A, Pape HC. Bone defects caused by high-energy injuries, bone loss, infected nonunions, and nonunions. Orthop Clin North Am, 2010, 41(1): 1-4.
3.	Hesse E, Kluge G, Atfi A, et al. Repair of a segmental long bone defect in human by implantation of a novel multiple disc graft. Bone, 2010, 46(5): 1457-1463.
4.	Meinel L, Fajardo R, Hofmann S, et al. Silk implants for the healing of critical size bone defects. Bone, 2005, 37(5): 688-698.
5.	Van de Watering FC, van den Beucken JJ, Walboomers XF, et al. Calcium phosphate/poly (D, L-lactic-co-glycolic acid) composite bone substitute materials: evaluation of temporal degradation and bone ingrowth in a rat critical-sized cranial defect. Clin Oral Implants Res, 2012, 23(2): 151-159.
6.	Brevi BC, Magri AS, Toma L, et al. Cranioplasty for repair of a large bone defect with autologous and homologous bone in children. J Pediatr Surg, 2010, 45(4): E17-20.
7.	Si HP, Lu ZH, Lin YL, et al. Transfect bone marrow stromal cells with pcDNA3.1-VEGF to construct tissue engineered bone in defect repair. Chin Med J (Engl), 2012, 125(5): 906-911.
8.	李福兵, 杜曉蘭, 余瑛, 等. 骨形成蛋白4條件性RNA干擾小鼠的建立. 遺傳, 2008, 30(3): 341-346.
9.	李福兵, 趙玲, 魯秀敏, 等. 成熟成骨細胞中敲除基因fgfr1小鼠的制備. 第三軍醫大學學報, 2008, 30(4): 280-283.
10.	湯譯博, 趙亮, 蘇佳燦. 骨折動物模型的研究進展. 中國骨傷, 2011, 24(1): 91-93.
11.	Monfoulet L, Rabier B, Chassande O, et al. Drilled hole defects in mouse femur as models of intramembranous cortical and cancellous bone regeneration. Calcif Tissue Int, 2010, 86(1): 72-81.
12.	Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat Res, 1986, (205): 299-308.
13.	Gokhale A, Rwigema JC, Epperly MW, et al. Small molecule GS-nitroxide ameliorates ionizing irradiation-induced delay in bone wound healing in a novel murine model. In Vivo, 2010, 24(4): 377-385.
14.	Kim MG, Shin DM, Lee SW. The healing of critical-sized bone defect of rat zygomatic arch with particulate bone graft and bone morphogenetic protein-2. J Plast Reconstr Aesthet Surg, 2010, 63(3): 459-466.
15.	de Girolamo L, Arrigoni E, Stanco D, et al. Role of autologous rabbit adipose-derived stem cells in the early phases of the repairing process of critical bone defects. J Orthop Res, 2011, 29(1): 100-108.
16.	靳慧勇, 侯天勇, 羅飛, 等. 小鼠股骨臨界骨缺損模型的構建及評估. 第三軍醫大學學報, 2011, 33(22): 2331-2334.
17.	Nagashima M, Sakai A, Uchida S, et al. Bisphosphonate (YM529) delays the repair of cortical bone defect after drill-hole injury by reducing terminal differentiation of osteoblasts in the mouse femur. Bone, 2005, 36(3): 502-511.
18.	Zhao M, Zhou J, Li X, et al. Repair of bone defect with vascularized tissue engineered bone graft seeded with mesenchymal stem cells in rabbits. Microsurgery, 2011, 31(2): 130-137.
19.	Anderson ML, Dhert WJ, de Bruijn JD, et al. Critical size defect in the goat’s os ilium. A model to evaluate bone grafts and substitutes. Clin Orthop Relat Res, 1999, (364): 231-239.
20.	Lian Z, Chuanchang D, Wei L, et al. Enhanced healing of goat femur-defect using BMP7 gene-modified BMSCs and load-bearing tissue-engineered bone. J Orthop Res, 2010, 28(3): 412-418.
21.	Riegger C, Kropil P, Jungbluth P, et al. Quantitative assessment of bone defect healing by multidetector CT in a pig model. Skeletal Radiol, 2012, 41(5): 531-537.
22.	Thoma DS, Halg GA, Dard MM, et al. Evaluation of a new biodegradable membrane to prevent gingival ingrowth into mandibular bone defects in minipigs. Clin Oral Implants Res, 2009, 20(1): 7-16.

1. Masquelet AC, Beque T. The concept of induced membrane for reconstruction of long bone defects. Orthop Clin North Am, 2010, 41(1): 27-37.
2. Wiese A, Pape HC. Bone defects caused by high-energy injuries, bone loss, infected nonunions, and nonunions. Orthop Clin North Am, 2010, 41(1): 1-4.
3. Hesse E, Kluge G, Atfi A, et al. Repair of a segmental long bone defect in human by implantation of a novel multiple disc graft. Bone, 2010, 46(5): 1457-1463.
4. Meinel L, Fajardo R, Hofmann S, et al. Silk implants for the healing of critical size bone defects. Bone, 2005, 37(5): 688-698.
5. Van de Watering FC, van den Beucken JJ, Walboomers XF, et al. Calcium phosphate/poly (D, L-lactic-co-glycolic acid) composite bone substitute materials: evaluation of temporal degradation and bone ingrowth in a rat critical-sized cranial defect. Clin Oral Implants Res, 2012, 23(2): 151-159.
6. Brevi BC, Magri AS, Toma L, et al. Cranioplasty for repair of a large bone defect with autologous and homologous bone in children. J Pediatr Surg, 2010, 45(4): E17-20.
7. Si HP, Lu ZH, Lin YL, et al. Transfect bone marrow stromal cells with pcDNA3.1-VEGF to construct tissue engineered bone in defect repair. Chin Med J (Engl), 2012, 125(5): 906-911.
8. 李福兵, 杜曉蘭, 余瑛, 等. 骨形成蛋白4條件性RNA干擾小鼠的建立. 遺傳, 2008, 30(3): 341-346.
9. 李福兵, 趙玲, 魯秀敏, 等. 成熟成骨細胞中敲除基因fgfr1小鼠的制備. 第三軍醫大學學報, 2008, 30(4): 280-283.
10. 湯譯博, 趙亮, 蘇佳燦. 骨折動物模型的研究進展. 中國骨傷, 2011, 24(1): 91-93.
11. Monfoulet L, Rabier B, Chassande O, et al. Drilled hole defects in mouse femur as models of intramembranous cortical and cancellous bone regeneration. Calcif Tissue Int, 2010, 86(1): 72-81.
12. Schmitz JP, Hollinger JO. The critical size defect as an experimental model for craniomandibulofacial nonunions. Clin Orthop Relat Res, 1986, (205): 299-308.
13. Gokhale A, Rwigema JC, Epperly MW, et al. Small molecule GS-nitroxide ameliorates ionizing irradiation-induced delay in bone wound healing in a novel murine model. In Vivo, 2010, 24(4): 377-385.
14. Kim MG, Shin DM, Lee SW. The healing of critical-sized bone defect of rat zygomatic arch with particulate bone graft and bone morphogenetic protein-2. J Plast Reconstr Aesthet Surg, 2010, 63(3): 459-466.
15. de Girolamo L, Arrigoni E, Stanco D, et al. Role of autologous rabbit adipose-derived stem cells in the early phases of the repairing process of critical bone defects. J Orthop Res, 2011, 29(1): 100-108.
16. 靳慧勇, 侯天勇, 羅飛, 等. 小鼠股骨臨界骨缺損模型的構建及評估. 第三軍醫大學學報, 2011, 33(22): 2331-2334.
17. Nagashima M, Sakai A, Uchida S, et al. Bisphosphonate (YM529) delays the repair of cortical bone defect after drill-hole injury by reducing terminal differentiation of osteoblasts in the mouse femur. Bone, 2005, 36(3): 502-511.
18. Zhao M, Zhou J, Li X, et al. Repair of bone defect with vascularized tissue engineered bone graft seeded with mesenchymal stem cells in rabbits. Microsurgery, 2011, 31(2): 130-137.
19. Anderson ML, Dhert WJ, de Bruijn JD, et al. Critical size defect in the goat’s os ilium. A model to evaluate bone grafts and substitutes. Clin Orthop Relat Res, 1999, (364): 231-239.
20. Lian Z, Chuanchang D, Wei L, et al. Enhanced healing of goat femur-defect using BMP7 gene-modified BMSCs and load-bearing tissue-engineered bone. J Orthop Res, 2010, 28(3): 412-418.
21. Riegger C, Kropil P, Jungbluth P, et al. Quantitative assessment of bone defect healing by multidetector CT in a pig model. Skeletal Radiol, 2012, 41(5): 531-537.
22. Thoma DS, Halg GA, Dard MM, et al. Evaluation of a new biodegradable membrane to prevent gingival ingrowth into mandibular bone defects in minipigs. Clin Oral Implants Res, 2009, 20(1): 7-16.

《中國修復重建外科雜志》

小鼠脛骨中段1/3不同缺損直徑單層骨皮質缺損模型比較研究

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《中國修復重建外科雜志》

小鼠脛骨中段1/3不同缺損直徑單層骨皮質缺損模型比較研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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