化學小分子reversine誘導細胞去分化的研究進展_《中國修復重建外科雜志》

作者：

胡旭琪 , 張小磊 , 周一飛 , 余洋 , 徐華梓

溫州醫學院附屬第二醫院骨科（浙江溫州，325000）;

關鍵詞：

多能干細胞 reversine 去分化重新編程

視頻：

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目的總結目前將成體細胞轉變成干細胞的常用技術，并對化學小分子reversine誘導細胞去分化的研究進展進行綜述。方法查閱近年來關于成體細胞轉變為干細胞及reversine誘導細胞去分化的相關文獻，并進行總結分析。結果通過體細胞核轉移技術、基因轉染技術等方法將成體細胞轉變成干細胞存在某些缺點，而reversine誘導細胞去分化具備獨特優勢，但其機制尚未完全明確。結論化學小分子reversine誘導細胞去分化有望成為誘導成體細胞轉變為干細胞的主要方法。

引用本文： 胡旭琪,張小磊,周一飛,余洋,徐華梓. 化學小分子reversine誘導細胞去分化的研究進展. 中國修復重建外科雜志, 2012, 26(9): 1126-1129. doi: 復制

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16.	Anastasia L, Sampaolesi M, Papini N, et al. Reversine-treated fibroblasts acquire myogenic competence in vitro and in regenerating skeletal muscle. Cell Death Differ, 2006, 13(12): 2042-2051.
17.	Saraiya M, Nasser R, Zeng Y, et al. Reversine enhances generation of progenitor-like cells by dedifferentiation of annulus fibrosus cells. Tissue Eng Part A, 2010, 16(4): 1443-1455.
18.	Lee EK, Bae GU, You JS, et al. Reversine increases the plasticity of lineage-committed cells toward neuroectodermal lineage. J Biol Chem, 2009, 284(5): 2891-2901.
19.	Bellefroid EJ, Kobbe A, Gruss P, et al. Xiro3 encodes a Xenopus homolog of the Drosophila Iroquois genes and functions in neural specification. EMBO J, 1998, 17(1): 191-203.
20.	Basch ML, Bronner-Fraser M, García-Castro MI. Specification of the neural crest occurs during gastrulation and requires Pax7. Nature, 2006, 441(7090): 218-222.
21.	Kageyama R, Ohtsuka T, Kobayashi T. The Hes gene family: repressors and oscillators that orchestrate embryogenesis. Development, 2007, 134(7): 1243-1251.
22.	Kim YK, Choi HY, Kim NH, et al. Reversine stimulates adipocyte differentiation and downregulates Akt and p70 (s6k) signaling pathways in 3T3-L1 cells. Biochem Biophys Res Commun, 2007, 358(2): 553-558.
23.	D’Alise AM, Amabile G, Iovino M, et al. Reversine, a novel Aurora kinases inhibitor, inhibits colony formation of human acute myeloid leukemia cells. Mol Cancer Ther, 2008, 7(5): 1140-1149.
24.	Vader G, Cruijsen CW, van Harn T, et al. The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule-kinetochore interactions. Mol Biol Cell, 2007, 18(11): 4553-4564.
25.	Musacchio A, Salmon ED. The spindle-assembly checkpoint in space and time. Nat Rev Mol Cell Biol, 2007, 8(5): 379-393.
26.	Amabile G, D’Alise AM, Iovino M, et al. The Aurora B kinase activity is required for the maintenance of the differentiated state of murine myoblasts. Cell Death Differ, 2009, 16(2): 321-330.
27.	Santaguida S, Tighe A, D’Alise AM, et al. Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J Cell Biol, 2010, 190(1): 73-87.
28.	Jung DW, Williams DR. Novel chemically defined approach to produce multipotent cells from terminally differentiated tissue syncytia. ACS Chem Biol, 2011, 6(6): 553-562.

1. Morrison SJ, Kimble J. Asymmetric and symmetric stem-cell divisions in development and cancer. Nature, 2006, 441(7097): 1068-1074.
2. Anastasia L, Piccoli M, Garatti A, et al. Cell reprogramming: a new chemical approach to stem cell biology and tissue regeneration. Curr Pharm Biotechnol, 2011, 12(2): 146-150.
3. Anastasia L, Pelissero G, Venerando B, et al. Cell reprogramming: expectations and challenges for chemistry in stem cell biology and regenerative medicine. Cell Death Differ, 2010, 17(8): 1230-1237.
4. Campbell KH, McWhir J, Ritchie WA, et al. Sheep cloned by nuclear transfer from a cultured cell line. Nature, 1996, 380(6569): 64-66.
5. Cowan CA, Atienza J, Melton DA, et al. Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells. Science, 2005, 309(5739): 1369-1373.
6. Tada M, Takahama Y, Abe K, et al. Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells. Curr Biol, 2001, 11(19): 1553-1558.
7. Takahashi K, Yamanaka S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell, 2006, 126(4): 663-676.
8. Lowry WE, Richter L, Yachechko R, et al. Generation of human induced pluripotent stem cells from dermal fibroblasts. Proc Natl Acad Sci U S A, 2008, 105(8): 2883-2888.
9. Okita K, Ichisaka T, Yamanaka S. Generation of germline-competent induced pluripotent stem cells. Nature, 2007, 448(7151): 313-317.
10. Mochiduki Y, Okita K. Methods for iPS cell generation for basic research and clinical applications. Biotechnology J, 2012, 7(6): 789-797.
11. Bain G, Kitchens D, Yao M, et al. Embryonic stem cells express neuronal properties in vitro. Dev Biol, 1995, 168(2): 342-357.
12. Christman JK. 5-Azacytidine and 5-aza-2’-deoxycytidine as inhibitors of DNA methylation: mechanistic studies and their implications for cancer therapy. Oncogene, 2002, 21(35): 5483-5495.
13. Esteban MA, Wang T, Qin BM, et al. Vitamin C enhances the generation of mouse and human induced pluripotent stem cells. Cell Stem Cell, 2010, 6(1): 71-79.
14. Chen S, Zhang Q, Wu X, et al. Dedifferentiation of lineage-committed cells by a small molecule. J Am Chem Soc, 2004, 126(2): 410-411.
15. Chen S, Takanashi S, Zhang Q, et al. Reversine increases the plasticity of lineage-committed mammalian cells. Proc Natl Acade Sci U S A, 2007, 104(25): 10482-10487.
16. Anastasia L, Sampaolesi M, Papini N, et al. Reversine-treated fibroblasts acquire myogenic competence in vitro and in regenerating skeletal muscle. Cell Death Differ, 2006, 13(12): 2042-2051.
17. Saraiya M, Nasser R, Zeng Y, et al. Reversine enhances generation of progenitor-like cells by dedifferentiation of annulus fibrosus cells. Tissue Eng Part A, 2010, 16(4): 1443-1455.
18. Lee EK, Bae GU, You JS, et al. Reversine increases the plasticity of lineage-committed cells toward neuroectodermal lineage. J Biol Chem, 2009, 284(5): 2891-2901.
19. Bellefroid EJ, Kobbe A, Gruss P, et al. Xiro3 encodes a Xenopus homolog of the Drosophila Iroquois genes and functions in neural specification. EMBO J, 1998, 17(1): 191-203.
20. Basch ML, Bronner-Fraser M, García-Castro MI. Specification of the neural crest occurs during gastrulation and requires Pax7. Nature, 2006, 441(7090): 218-222.
21. Kageyama R, Ohtsuka T, Kobayashi T. The Hes gene family: repressors and oscillators that orchestrate embryogenesis. Development, 2007, 134(7): 1243-1251.
22. Kim YK, Choi HY, Kim NH, et al. Reversine stimulates adipocyte differentiation and downregulates Akt and p70 (s6k) signaling pathways in 3T3-L1 cells. Biochem Biophys Res Commun, 2007, 358(2): 553-558.
23. D’Alise AM, Amabile G, Iovino M, et al. Reversine, a novel Aurora kinases inhibitor, inhibits colony formation of human acute myeloid leukemia cells. Mol Cancer Ther, 2008, 7(5): 1140-1149.
24. Vader G, Cruijsen CW, van Harn T, et al. The chromosomal passenger complex controls spindle checkpoint function independent from its role in correcting microtubule-kinetochore interactions. Mol Biol Cell, 2007, 18(11): 4553-4564.
25. Musacchio A, Salmon ED. The spindle-assembly checkpoint in space and time. Nat Rev Mol Cell Biol, 2007, 8(5): 379-393.
26. Amabile G, D’Alise AM, Iovino M, et al. The Aurora B kinase activity is required for the maintenance of the differentiated state of murine myoblasts. Cell Death Differ, 2009, 16(2): 321-330.
27. Santaguida S, Tighe A, D’Alise AM, et al. Dissecting the role of MPS1 in chromosome biorientation and the spindle checkpoint through the small molecule inhibitor reversine. J Cell Biol, 2010, 190(1): 73-87.
28. Jung DW, Williams DR. Novel chemically defined approach to produce multipotent cells from terminally differentiated tissue syncytia. ACS Chem Biol, 2011, 6(6): 553-562.

《中國修復重建外科雜志》

化學小分子reversine誘導細胞去分化的研究進展

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《中國修復重建外科雜志》

化學小分子reversine誘導細胞去分化的研究進展

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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