Ras同源類似物小干擾RNA對乳腺癌細胞惡性表型的影響_《華西醫學》

作者：

朱敏 ¹ , 夏洪偉 ² , 唐秋琳 ³ ,  畢鋒 ⁴

四川大學（成都，610041） 1華西臨床醫學院，2 華西醫院生物治療國家重點實驗室，3 華西醫院信號轉導及分子靶向治療研究室，4 華西醫院腫瘤科;

關鍵詞：

Ras同源類似物E 小干擾RNA 乳腺癌細胞惡性表型

視頻：

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目的　觀察下調Ras同源類似物E (RhoE)表達對人乳腺癌細胞231生物學行為的影響。方法　蛋白質印跡技術檢測小干擾RNA（siRNA）轉染前后RhoE在乳腺癌細胞231中的表達；RhoE siRNA的細胞轉染用lipofectamine?2000脂質體法；Cell Counting Kit-8檢測轉染細胞及對照細胞的增殖變化；損傷刮擦試驗和體外侵襲實驗（Transwell小室）分別檢測轉染細胞及對照細胞的遷移與侵襲能力。結果　RhoE在乳腺癌細胞231中的表達較高；成功轉染RhoE siRNA的乳腺癌細胞，蛋白質印跡顯示RhoE的表達被明顯的抑制；RhoE的表達被抑制后對乳腺癌細胞的增殖、遷移和侵襲有著明顯的促進作用。結論　下調RhoE 表達能夠明顯促進乳腺癌細胞的增殖﹑遷移和侵襲，RhoE可能在乳腺癌的發生發展中起著重要作用。

引用本文： 朱敏,夏洪偉,唐秋琳,畢鋒. Ras同源類似物小干擾RNA對乳腺癌細胞惡性表型的影響. 華西醫學, 2012, 27(4): 485-490. doi: 復制

1.	Vega FM, Ridley AJ. Rho GTPases in cancer cell biology[J]. FEBS Letters, 2008, 582 (14) : 2093-2101.
2.	Boureux A, Vignal E, Faure S, et al. Evolution of the Rho family of ras-like GTPases in eukaryotes[J]. Mol Biol Evol, 2007, 24(1): 203-216.
3.	Boettner B, Van Aelst L. The role of Rho GTPases in disease development[J]. Gene, 2002, 286(2): 155-174.
4.	Villalonga P, Guasch RM, Riento K, et al. RhoE inhibits cell cycle progression and Ras-induced transformation[J]. Mol Cell Biol, 2004, 24(18): 7829-7840.
5.	Roberts PJ, Mitin N, Keller PJ, et al. Rho Family GTPase modification and dependence on CAAX motif-signaled posttranslational modification[J]. J Biol Chem, 2008, 283(37): 25150-25163.
6.	Madigan JP, Bodemann BO, Brady DC，et al. Regulation of Rnd3 localization and function by protein kinase C alpha-mediated phosphorylation[J]. Biochem J, 2009, 424(1): 153-161.
7.	Riou P, Villalonga P, Ridley AJ. Rnd proteins: multifunctional regulators of the cytoskeleton and cell cycle progression[J]. Bioessays, 2010, 32(11): 986-992.
8.	Cuiyan Z, Jie H, Fang Z, et al.Overexpression of RhoE in non-small cell lung cancer (nsclc) is associated with smoking and correlates with DNA copy number changes[J]. Cancer Biol Ther, 2007, 6(3): 335-342.
9.	Klein1 RM, Andrew AE. Rnd3 regulation of the actin cytoskeleton promotes melanoma migration and invasive outgrowth in 3-D[J]. Cancer Res, 2009, 69(6): 2224-2233.
10.	Rubenstein NM, Chan JF, Kim JY, et al. Rnd3/RhoE induces tight junction formation in mammary epithelial tumor cells[J]. Exp Cell Res, 2005, 305(1): 74-82.
11.	Luo H, Zou J, Dong Z, et al. Up-regulated miR-17 promotes cell proliferation, tumour growth and cell cycle progression by targeting the RND3 tumour suppressor gene in colorectal carcinoma[J]. Biochem J, 2012, 442(2): 311-321.
12.	Chen J, Zhou H, Li Q, et al. Epigenetic modification of RhoE expression in gastric cancer cells[J]. Oncol Rep, 2011, 25(1): 173-180.
13.	Grise F, Sena S, Bidaud-Meynard A. Rnd3/RhoE is down-regulated in hepatocellular carcinoma and controls cellular invasion[J]. Hepatology, 2012, Jan 11. doi: 10.1002/hep.25568.
14.	Bektic J, Pfeil K, Berger AP, et al. Small G-protein RhoE is underexpressed in prostate cancer and induces cell cycle arrest and apoptosis[J]. Prostate, 2005, 64(4): 332-340.
15.	Li K, Lu Y, Liang J, et al. RhoE enhances multidrug resistance of gastric cancer cells by suppressing Bax[J]. Biochem Biophys Res Commun, 2009, 379(2): 212-216.
16.	Belgiovine C, Frapolli R, Bonezzi K, et al. Reduced expression of the ROCK inhibitor Rnd3 is associated with increased invasiveness and metastatic potential in mesenchymal tumor cells[J]. PLoS One, 2010, 5(11): e14154.

1. 　Vega FM, Ridley AJ. Rho GTPases in cancer cell biology[J]. FEBS Letters, 2008, 582 (14) : 2093-2101.
2. 　Boureux A, Vignal E, Faure S, et al. Evolution of the Rho family of ras-like GTPases in eukaryotes[J]. Mol Biol Evol, 2007, 24(1): 203-216.
3. 　Boettner B, Van Aelst L. The role of Rho GTPases in disease development[J]. Gene, 2002, 286(2): 155-174.
4. 　Villalonga P, Guasch RM, Riento K, et al. RhoE inhibits cell cycle progression and Ras-induced transformation[J]. Mol Cell Biol, 2004, 24(18): 7829-7840.
5. 　Roberts PJ, Mitin N, Keller PJ, et al. Rho Family GTPase modification and dependence on CAAX motif-signaled posttranslational modification[J]. J Biol Chem, 2008, 283(37): 25150-25163.
6. 　Madigan JP, Bodemann BO, Brady DC，et al. Regulation of Rnd3 localization and function by protein kinase C alpha-mediated phosphorylation[J]. Biochem J, 2009, 424(1): 153-161.
7. 　Riou P, Villalonga P, Ridley AJ. Rnd proteins: multifunctional regulators of the cytoskeleton and cell cycle progression[J]. Bioessays, 2010, 32(11): 986-992.
8. 　Cuiyan Z, Jie H, Fang Z, et al.Overexpression of RhoE in non-small cell lung cancer (nsclc) is associated with smoking and correlates with DNA copy number changes[J]. Cancer Biol Ther, 2007, 6(3): 335-342.
9. 　Klein1 RM, Andrew AE. Rnd3 regulation of the actin cytoskeleton promotes melanoma migration and invasive outgrowth in 3-D[J]. Cancer Res, 2009, 69(6): 2224-2233.
10. 　Rubenstein NM, Chan JF, Kim JY, et al. Rnd3/RhoE induces tight junction formation in mammary epithelial tumor cells[J]. Exp Cell Res, 2005, 305(1): 74-82.
11. 　Luo H, Zou J, Dong Z, et al. Up-regulated miR-17 promotes cell proliferation, tumour growth and cell cycle progression by targeting the RND3 tumour suppressor gene in colorectal carcinoma[J]. Biochem J, 2012, 442(2): 311-321.
12. 　Chen J, Zhou H, Li Q, et al. Epigenetic modification of RhoE expression in gastric cancer cells[J]. Oncol Rep, 2011, 25(1): 173-180.
13. 　Grise F, Sena S, Bidaud-Meynard A. Rnd3/RhoE is down-regulated in hepatocellular carcinoma and controls cellular invasion[J]. Hepatology, 2012, Jan 11. doi: 10.1002/hep.25568.
14. 　Bektic J, Pfeil K, Berger AP, et al. Small G-protein RhoE is underexpressed in prostate cancer and induces cell cycle arrest and apoptosis[J]. Prostate, 2005, 64(4): 332-340.
15. 　Li K, Lu Y, Liang J, et al. RhoE enhances multidrug resistance of gastric cancer cells by suppressing Bax[J]. Biochem Biophys Res Commun, 2009, 379(2): 212-216.
16. 　Belgiovine C, Frapolli R, Bonezzi K, et al. Reduced expression of the ROCK inhibitor Rnd3 is associated with increased invasiveness and metastatic potential in mesenchymal tumor cells[J]. PLoS One, 2010, 5(11): e14154.

《華西醫學》

Ras同源類似物小干擾RNA對乳腺癌細胞惡性表型的影響

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《華西醫學》

Ras同源類似物小干擾RNA對乳腺癌細胞惡性表型的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料