腺苷2A受體激活減輕小體積肝移植中的氧化應激反應_《中國普外基礎與臨床雜志》

作者：

朱劍飛 ¹ , 湯黎明 ² , 李德春 ¹

1.蘇州大學醫學院附屬第一醫院普外科(江蘇蘇州 215006);;
2.常州第二人民醫院普外科(江蘇常州 213003);

關鍵詞：

腺苷受體氧化應激缺血再灌注損傷小體積肝移植

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目的通過大鼠小體積肝移植模型研究腺苷2A受體(adenosine 2A receptor,A2AR)激動對氧化應激反應的影響。
方法將18只Lewis大鼠建立的小體積肝移植受體模型隨機均分為對照組、激動組及激動+拮抗組,并于再灌注后6 h分別檢測血漿丙氨酸氨基轉移酶(aspartate aminotransferase,AST)、酶類抗氧化物〔超氧化物歧化酶(superoxide dismutase,SOD)、過氧化氫酶(catalase,CAT)、谷胱苷肽過氧化物酶(glutathione peroxidase,GSH-Px)〕及非酶類抗氧化物〔抗壞血酸(ascorbic acid,AA)、谷胱苷肽(glutathione,GSH)、α生育酚(αtocopherol,TOC)〕和丙二醛(malondialdehyde,MDA)的水平。
結果與對照組及激動+拮抗組比較，激動組SOD和GSH-Px活性均明顯增加(P lt;0.05)，而CAT活性無明顯變化(P gt;0.05); MDA含量及AST水平均顯著下降(P lt;0.05); AA、GSH、TOC水平均顯著提高(P lt;0.05)。
結論 A2AR激活明顯增加了肝臟酶類抗氧化物活性，上調了非酶類抗氧化物的表達，抑制了毒性抗氧化產物MDA含量及AST的水平上升，提示A2AR激活抑制了脂質氧化損傷，增強了抗氧化能力，從而減少了在小體積肝移植中的缺血再灌注損傷。

引用本文： 朱劍飛,湯黎明,李德春. 腺苷2A受體激活減輕小體積肝移植中的氧化應激反應. 中國普外基礎與臨床雜志, 2010, 17(3): 243-247. doi: 復制

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12.	孫要武, 董艷梅, 吳嘉慧, 等. 復合維生素B 和谷胱甘肽對脂肪肝的保護作用 [J]. 衛生研究, 2009; 38(4): 413416.
13.	Kang P, Dalvie D, Smith E, et al. Bioactivation of lumiracoxib by peroxidases and human liver microsomes: identification of multiple quinone imine intermediates and GSH adducts [J]. Chem Res Toxicol, 2009; 22(1): 106117.
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17.	Traber MG, Atkinson J. Vitamin E, antioxidant and nothing more [J]. Free Radic Biol Med, 2007; 43(1): 415.
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19.	Shang F, Lu M, Dudek E, et al. Vitamin C and vitamin E restore the resistance of GSHdepleted lens cells to H2O2 [J]. Free Radic Biol Med, 2003; 34(5): 521530.
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21.	Reiter RJ, Tan DX, Manchester LC, et al. Biochemical reactivity of melatonin with reactive oxygen and nitrogen species: a review of the evidence [J]. Cell Biochem Biophys, 2001; 34(2): 237256.
22.	Frank J, Lundh T, Parker RS, et al. Dietary (+) catechin and BHT markedly increase αtocopherol concentrations in rats by a tocopherolωhydroxylaseindependent mechanism [J]. J Nutr, 2003; 133(10): 31953199.
23.	Ramanathan K, Shila S, Kumaran S, et al. Protective role of ascorbic acid and alphatocopherol on arsenicinduced microsomal dysfunctions [J]. Hum Exp Toxicol, 2003; 22(3): 129136.
24.	Evans ZP, Ellett JD, Fariss MW, et al. Vitamin E succinate reduces ischemia/reperfusion injury in steatotic livers [J]. Transplant Proc, 2008; 40(10): 33273329.
25.	Hiroyuki A. Molecular mechanisms of αtocopherol transfer protein (αTTP) dependent αtocopherol transfer in hepatocytes [J]. Jpn J Physiol, 2005; 55(Suppl): S31.

1. Ikegami T, Shimada M, Imura S, et al. Current concept of smallforsize grafts in living donor liver transplantation [J]. Surg Today, 2008; 38(11): 971982.
2. Song J, Zhang YW, Yao AH, et al. Adenoviral cardiotrophin1 transfer improves survival and early graft function after ischemia and reperfusion in rat smallforsize liver transplantation model [J]. Transpl Int， 2008; 21(4): 372383.
3. Yao A, Li X, Pu L, et al. Impaired hepatic regeneration by ischemic preconditioning in a rat model of smallforsize liver transplantation [J]. Transpl Immunol, 2007; 18(1): 3743.
4. Cederbaum AI, Lu Y, Wu D. Role of oxidative stress in alcoholinduced liver injury [J]. Arch Toxicol, 2009; 83(6):519548.
5. Sucher R, Gehwolf P, Kaier T, et al. Intracellular signaling pathways control mitochondrial events associated with the development of ischemia/reperfusionassociated damage [J]. Transpl Int, 2009; 22(9): 922930.
6. Tang LM, Wang YP, Wang K, et al. Protective effect of adenosine A2A receptor activation in smallforsize liver transplantation [J]. Transpl Int, 2007; 20(1): 93101.
7. Uchiyama S, Shimizu T, Shirasawa T. CuZnSOD deficiency causes ApoB degradation and induces hepatic lipid accumulation by impaired lipoprotein secretion in mice [J]. J Biol Chem, 2006; 281(42): 3171331719.
8. Décordé K, Ventura E, Lacan D, et al. An SOD rich melon extract Extramel prevents aortic lipids and liver steatosis in dietinduced model of atherosclerosis [J]. Nutr Metab Cardiovasc Dis, 2009 Aug 18. DOI:10.1016/j.numecd.2009.04.017.
9. 盧峻, 陳英松, 阿古拉, 等. 蒙醫溫針對大鼠疲勞模型肝臟MDA、GSH 含量及GSHPX、SOD活性的影響 [J]. 針刺研究, 2007; 32(3): 167169.
10. Kim SJ, Lee JW, Jung YS, et al. Ethanolinduced liver injury and changes in sulfur amino acid metabolomics in glutathione peroxidase and catalase double knockout mice [J]. J Hepatol, 2009; 50(6): 11841191.
11. Jiang WW, Kong LB, Li GQ, et al. Expression of iNOS in early injury in a rat model of smallforsize liver transplantation [J]. Hepatobiliary Pancreat Dis Int, 2009; 8(2): 146151.
12. 孫要武, 董艷梅, 吳嘉慧, 等. 復合維生素B 和谷胱甘肽對脂肪肝的保護作用 [J]. 衛生研究, 2009; 38(4): 413416.
13. Kang P, Dalvie D, Smith E, et al. Bioactivation of lumiracoxib by peroxidases and human liver microsomes: identification of multiple quinone imine intermediates and GSH adducts [J]. Chem Res Toxicol, 2009; 22(1): 106117.
14. Crincoli CM, Patel NN, Harvison PJ. Formation of glutathione (GSH) conjugates from 3(3, 5dichlorophenyl) 2, 4thiazolidinedione (DCPT) by rat liver microsomes [J]. FASEB J, 2007; 21: 731.2.
15. Chou PT, Khan AU. Lascorbic acid quenching of singlet delta molecular oxygen in aqueous media: generalized antioxidant property of vitamin C [J]. Biochem Biophys Res Commun, 1983; 115(3): 932937.
16. Dillard CJ, Gavino VC, Tappel AL. Relative antioxidant effectiveness of αtocopherol and ωtocopherol in ironloaded rats [J]. J Nutr, 1983; 113(11): 22662273.
17. Traber MG, Atkinson J. Vitamin E, antioxidant and nothing more [J]. Free Radic Biol Med, 2007; 43(1): 415.
18. Soden JS, Devereaux MW, Haas JE, et al. Subcutaneous vitamin E ameliorates liver injury in an in vivo model of steatocholestasis [J]. Hepatology, 2007; 46(2): 485495.
19. Shang F, Lu M, Dudek E, et al. Vitamin C and vitamin E restore the resistance of GSHdepleted lens cells to H2O2 [J]. Free Radic Biol Med, 2003; 34(5): 521530.
20. Ross D. Glutathione, free radicals and chemotherapeutic agents. Mechanisms of freeradical induced toxicity and glutathionedependent protection [J]. Pharmacol Ther, 1988; 37(2): 231249.
21. Reiter RJ, Tan DX, Manchester LC, et al. Biochemical reactivity of melatonin with reactive oxygen and nitrogen species: a review of the evidence [J]. Cell Biochem Biophys, 2001; 34(2): 237256.
22. Frank J, Lundh T, Parker RS, et al. Dietary (+) catechin and BHT markedly increase αtocopherol concentrations in rats by a tocopherolωhydroxylaseindependent mechanism [J]. J Nutr, 2003; 133(10): 31953199.
23. Ramanathan K, Shila S, Kumaran S, et al. Protective role of ascorbic acid and alphatocopherol on arsenicinduced microsomal dysfunctions [J]. Hum Exp Toxicol, 2003; 22(3): 129136.
24. Evans ZP, Ellett JD, Fariss MW, et al. Vitamin E succinate reduces ischemia/reperfusion injury in steatotic livers [J]. Transplant Proc, 2008; 40(10): 33273329.
25. Hiroyuki A. Molecular mechanisms of αtocopherol transfer protein (αTTP) dependent αtocopherol transfer in hepatocytes [J]. Jpn J Physiol, 2005; 55(Suppl): S31.

《中國普外基礎與臨床雜志》

腺苷2A受體激活減輕小體積肝移植中的氧化應激反應

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