7種microRNAs在原發性肝癌組織和癌旁組織間的差異表達及與血清腫瘤標志物水平的相關性研究_《中國普外基礎與臨床雜志》

作者：

張明 , 劉衛輝 , 尤楠 , 張福琴 , 竇科峰

第四軍醫大學西京醫院肝膽外科(西安 710032);

關鍵詞：

小RNA MiR-200a 原發性肝細胞癌甲胎蛋白

視頻：

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目的分析小RNA（microRNAs）在原發性肝細胞癌組織和癌旁組織中的表達差異及與血清腫瘤標志物水平的相關性，探索肝癌診斷和預后可能的新標志。方法使用實時定量PCR技術檢測7種MicroRNAs在原發性肝細胞癌組織和癌旁組織中的表達差異。結果 7種microRNAs在原發性肝細胞癌組織和癌旁組織中的表達差異均有統計學意義(P lt;0.05)。與癌旁組織相比，miR-34c、miR-21、miR-16及miR-10b在肝癌組織中表達上調，miR-200a、miR-148b及miR-Let-7i在肝癌組織中表達下調，其中miR-200a 和 miR-148b下調明顯。miR-200a在肝癌和癌旁組織中的表達差異與患者血清AFP水平呈正相關(r=0.848 9，P lt;0.01)，而其余microRNAs的表達差異與患者血清各腫瘤標志物水平均無相關關系（P gt;0.05）。結論原發性肝細胞癌和癌旁組織中存在microRNAs的表達差異，miR-200a可能成為新的肝癌早期診斷標志。

引用本文： 張明,劉衛輝,尤楠,張福琴,竇科峰. 7種microRNAs在原發性肝癌組織和癌旁組織間的差異表達及與血清腫瘤標志物水平的相關性研究. 中國普外基礎與臨床雜志, 2010, 17(6): 562-566. doi: 復制

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24.	勞明，朱波，黃玲莎，等．三種腫瘤標志物聯合檢測對原發性肝癌的診斷價值 [J]．中華消化雜志， 2005; 25(2)： 109-110．.
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1. Kim VN, Nam JW. Genomics of microRNA [J]. Trends Genet, 2006; 22(3): 165-173.
2. Ke XS, Liu CM, Liu DP, et al. MicroRNAs: key participants in gene regulatory networks [J]. Curr Opin Chem Biol, 2003; 7(4): 516-523.
3. Dillhoff M, Wojcik SE, Bloomston M. MicroRNAs in solid tumors [J]. J Surg Res, 2009; 154(2): 349-354.
4. Osada H, Takahashi T. MicroRNAs in biological processes and carcinogenesis [J]. Carcinogenesis, 2007; 28(1): 2-12.
5. Lagos-Quintana M, Rauhut R, Lendeckel W, et al. Identification of novel genes coding for small expressed RNAs [J]. Science, 2001; 294(5543): 853-858.
6. Reinhart BJ, Weinstein EG, Rhoades MW, et al. Micro-RNAs in plants [J]. Genes Dev, 2002; 16(13): 1616-1626.
7. Ambros V. The functions of animal microRNAs [J]. Nature, 2004; 431(7006): 350-355.
8. Jin P, Alisch RS, Warren ST. RNA and microRNAs in fragile X mental retardation [J]. Nat Cell Biol, 2004; 6(11): 1048-1053.
9. Gregory RI, Yan KP, Amuthan G, et al. The Microprocessor complex mediates the genesis of microRNAs [J]. Nature, 2004; 432(7014): 235-240.
10. McGlynn KA, Tsao L, Hsing AW, et al. International trends and patterns of primary liver cancer [J]. Int J Cancer, 2001; 94(2): 290-296.
11. Murakami Y, Yasuda T, Saigo K, et al. Comprehensive analysis of microRNA expression patterns in hepatocellular carcinoma and non-tumorous tissues [J]. Oncogene, 2006; 25(17): 2537-2545.
12. 劉衛輝, 吳印濤, 李韌，等. 大鼠肝癌細胞與胎肝細胞間抑癌微小RNAs的表達差異 [J]. 中華肝臟病雜志, 2009; 17（2）: 75-77.
13. Lu J, Getz G, Miska EA, et al. MicroRNA expression profiles classify human cancers [J]. Nature, 2005; 435(7043): 834-838.
14. Horie Y, Suzuki A, Kataoka E, et al. Hepatocyte-specific Pten deficiency results in steatohepatitis and hepatocellular carcinomas [J]. J Clin Invest, 2004; 113(12): 1774-1783.
15. Meng F, Henson R, Lang M, et al. Involvement of human micro-RNA in growth and response to chemotherapy in human cholangiocarcinoma cell lines [J]. Gastroenterology, 2006; 130(7): 2113-2129.
16. Okamura K, Ishizuka A, Siomi H, et al. Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways [J]. Genes Dev, 2004; 18(14): 1655-1666.
17. Esquela-Kerscher A, Slack FJ. Oncomirs-microRNAs with a role in cancer [J]. Nat Rev Cancer, 2006; 6(4): 259-269.
18. Johnson SM, Grosshans H, Shingara J, et al. RAS is regulated by the let-7 microRNA family [J]. Cell, 2005; 120(5): 635-647.
19. O’Donnell KA, Wentzel EA, Zeller KI, et al. c-Myc-regulated microRNAs modulate E2F1 expression. [J]. Nature, 2005; 435(7043): 839-843.
20. Budhu A, Jia HL, Forgues M, et al. Identification of metastasis-related microRNAs in hepatocellular carcinoma [J]. Hepatology, 2008; 47(3): 897-907.
21. Gregory PA, Bert AG, Paterson EL, et al. The miR-200 family and miR-205 regulate epithelial to mesenchymal transition by targeting ZEB1 and SIP1 [J]. Nat Cell Biol, 2008; 10(5): 593-601.
22. Taketa K. Alpha-fetoprotein: reevaluation in hepatology [J]. Hepatology, 1990; 12(6): 1420-1432.
23. 邵浙新，徐驍，鄭樹森，等．肝細胞癌患者肝移植術后甲胎蛋白的變化與腫瘤復發 [J]．中華普通外科雜志， 2006; 21(5)： 351-353．.
24. 勞明，朱波，黃玲莎，等．三種腫瘤標志物聯合檢測對原發性肝癌的診斷價值 [J]．中華消化雜志， 2005; 25(2)： 109-110．.
25. 馬京香, 宮奇林, 林梅青，等．多項腫瘤標志物聯合檢測早期診斷原發性肝癌的研究 [J]．中華外科雜志， 2000; 38(1)： 14-16．.

《中國普外基礎與臨床雜志》

7種microRNAs在原發性肝癌組織和癌旁組織間的差異表達及與血清腫瘤標志物水平的相關性研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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《中國普外基礎與臨床雜志》

7種microRNAs在原發性肝癌組織和癌旁組織間的差異表達及與血清腫瘤標志物水平的相關性研究

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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