VEGF參與低氧預適應對移植肝肝內膽管上皮細胞的保護作用_《中國普外基礎與臨床雜志》

作者：

周斌 , 張培建 , 李勇 , 田田 , 金成 , 劉新顏 , 馮敏 , 郎潔

揚州大學第二臨床醫學院普通外科研究室（江蘇揚州 225001）;

關鍵詞：

血管內皮生長因子低氧預適應肝移植膽管上皮細胞

視頻：

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摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的探討低氧預適應（hypoxic preconditioning，HP）對移植肝肝內膽管上皮細胞的保護作用及血管內皮生長因子（VEGF）在其中的作用。方法建立大鼠自體原位肝移植模型，實驗大鼠被分為3組：自體原位肝移植組(AT組)、HP后行自體原位肝移植組（HP組）和假手術組。各組于術后6、12、24和48 h檢測血清總膽紅素（TBIL）、直接膽紅素（DBIL）及堿性磷酸酶（ALP）水平，免疫組織化學法檢測肝內膽管上皮細胞中VEGF的表達，光鏡下觀察肝內膽管上皮細胞的病理變化。結果 AT組術后血清TBIL、DBIL及ALP水平持續高于HP組（P＜0.05）；各時相HP組VEGF的表達均明顯高于AT組（P＜0.05）；光鏡下，HP組各時相肝內膽管上皮細胞損傷及炎癥細胞浸潤程度均較AT組明顯減輕。結論 HP對移植肝肝內膽管上皮細胞有保護作用，VEGF可能在其中發揮著重要作用

引用本文： 周斌,張培建,李勇,田田,金成,劉新顏,馮敏,郎潔. VEGF參與低氧預適應對移植肝肝內膽管上皮細胞的保護作用. 中國普外基礎與臨床雜志, 2009, 16(5): 365-369. doi: 復制

1.	Wang Y, Mao XO, Xie L, et al. Vascular endothelial growth factor overexpression delays neurodegeneration and prolongs survival in amyotrophic lateral sclerosis mice [J]. J Neurosci, 2007; 27 (2): 304-307.
2.	Li LH, Guo ZJ, Yan LL, et al． Antitumor and antiangiogenic activities of anti-vascular endothelial growth factor hairpin ribozyme in human hepatocellular carcinoma cell cultures and xenografts [J]. World J Gastroenterol, 2007; 13(47): 6425-6432.
3.	Stroka DM, Burkhardt T, Desbaillets I, et al. HIF-1 is expressed in normoxic tissue and displays an organ specific regulation under systemic hypoxia [J]. FASEB J, 2001; 15(13): 2445-2453.
4.	陶立德，張培建, 田明祥, 等. 低氧預適應對移植肝臟肝竇內皮細胞損傷的影響 [J]. 實用臨床醫藥雜志, 2007; 11(2): 39-42.
5.	趙宏峰, 周杰. 大鼠自體原位肝移植膽道缺血再灌注損傷模型的建立 [J]. 第二軍醫大學學報, 2006； 27(4): 429-430.
6.	梁四海, 陶立德, 張培建, 等. 低氧預適應對大鼠移植肝臟功能和組織結構的影響 [J]. 江蘇大學學報(醫學版), 2007; 17(3): 219-221.
7.	張培建, 卜平, 金成. 低氧預適應對移植大鼠肝細胞線粒體超微結構的影響 [J]. 解剖學報, 2008; 39(6): 854-857.
8.	孔連寶, 吳曉峰, 邵立軍, 等. 吻合肝動脈對大鼠部分肝移植早期損傷的影響 [J]. 中華實驗外科雜志, 2006; 23(5): 543-545.
9.	彭方興, 鄭本波, 劉立新, 等. 大鼠原位肝移植動物模型制作要點 [J]. 中國普外基礎與臨床雜志, 2005; 12(2）: 117-119.
10.	李勇, 張培建. 肝移植術后免疫排斥反應和肝功能監測 [J]. 醫學研究生學報, 2008； 21(3): 313-316.
11.	易述紅, 陳規劃, 陸敏強, 等. VEGF mRNA在預測肝癌肝移植術后腫瘤復發和轉移中的應用價值 [J]. 中國普外基礎與臨床雜志, 2006; 13(5): 560-564.
12.	Christou H, Yoshida A, Arthur V, et al． Increased vascular endothelial growth factor production in the lungs of rats with hypoxia-induced pulmonary hypertension [J]. Am J Respir Cell Mol Biol, 1998; 18(6): 768-776.
13.	Liu LX, Lu H, Luo Y, et al. Stabilization of vascular endothelial growth factor mRNA by hypoxia-inducible factor 1 [J]. Biochem Biophys Res Commum, 2002; 291(4): 908-914.
14.	李興睿, 廖曉峰, 易繼林. 缺氧對肝癌細胞系HepG2表達VEGF的影響 [J]. 中國普外基礎與臨床雜志, 2005; 12(5): 477-479.
15.	Shibuya M, Claesson-Welsh L. Signal transduction by VEGF receptors in regulation of angiogenesis and lymphangiogenesis [J]. Exp Cell Res, 2006; 312(5): 549-560.
16.	Wilkinson-Berka JL. Vasoactive factors and diabetic retinopathy: vascular endothelial growth factor, cycoloxygenase-2 and nitric oxide [J]. Curr Pharm Des, 2004; 10(27): 3331-3348.
17.	Teraoka H, Sawada T, Nishihara T, et al. Enhanced VEGF production and decreased immunogenicity induced by TGF beta1 promote liver metastasis of pancreatic cancer [J]. Br J Cancer, 2001; 85(4): 612-617.
18.	Zuo S, Ji Y, Wang J, et al. Expression and clinical implication of HIF-1alpha and VEGF-C in non-small cell lung cancer [J]. J Huazhong Univ Sci Technolog Med Sci, 2008; 28(6): 674-676.
19.	Gaudio E, Barbaro B, Alvaro D, et al. Vascular endothelial growth factor stimulates rat cholangiocyte proliferation via an autocrine mechanism [J]. Gastroenterology, 2006; 130(4): 1270-1282.
20.	Gaudio E, Barbara Bo, Alvaro D, et al. Administration of r-VEGF-A prevents hepatic artery ligation-induced bile duct damage in bile duct ligated rats [J]. Am J Physiol Gastrointest Liver Physiol, 2006; 291(2): G307-G317.
21.	Xu WH, Ye QF, Xia SS. Apoptosis and proliferation of intrahepatic bile duct after ischemia-reperfusion injury [J]. Hepatobiliary Pancreat Dis Int, 2004; 3(3): 428-432.
22.	李文崗, 胡素賢, 段偉東, 等. 完全失去動脈血供后大鼠肝門部膽管微血管數對膽管上皮細胞核增殖與ER表達影響 [J]. 中華肝膽外科雜志, 2007; 13(8): 548-550.
23.	Gaudio E, Onori P, Franchitto A, et al. Hepatic microcirculation and cholangiocyte physiopathology [J]. Ital J Anat Embryol, 2005; 110(2 Suppl 1): 71-75.
24.	Bissinger AL, Oettle H, Jahn G, et al. Cytomegalovirus infection after orthotopic liver transplantation is restricted by a pre-existing antiviral immune response of the recipient [J]. J Med Virol, 2004; 73(1): 45-53.
25.	Beaussier M, Wendum D, Fouassier L, et al. Adaptative bile duct proliferative response in experimental bile duct ischemia [J]. J Hepatol, 2005; 42(2): 257-265.

1. Wang Y, Mao XO, Xie L, et al. Vascular endothelial growth factor overexpression delays neurodegeneration and prolongs survival in amyotrophic lateral sclerosis mice [J]. J Neurosci, 2007; 27 (2): 304-307.
2. Li LH, Guo ZJ, Yan LL, et al． Antitumor and antiangiogenic activities of anti-vascular endothelial growth factor hairpin ribozyme in human hepatocellular carcinoma cell cultures and xenografts [J]. World J Gastroenterol, 2007; 13(47): 6425-6432.
3. Stroka DM, Burkhardt T, Desbaillets I, et al. HIF-1 is expressed in normoxic tissue and displays an organ specific regulation under systemic hypoxia [J]. FASEB J, 2001; 15(13): 2445-2453.
4. 陶立德，張培建, 田明祥, 等. 低氧預適應對移植肝臟肝竇內皮細胞損傷的影響 [J]. 實用臨床醫藥雜志, 2007; 11(2): 39-42.
5. 趙宏峰, 周杰. 大鼠自體原位肝移植膽道缺血再灌注損傷模型的建立 [J]. 第二軍醫大學學報, 2006； 27(4): 429-430.
6. 梁四海, 陶立德, 張培建, 等. 低氧預適應對大鼠移植肝臟功能和組織結構的影響 [J]. 江蘇大學學報(醫學版), 2007; 17(3): 219-221.
7. 張培建, 卜平, 金成. 低氧預適應對移植大鼠肝細胞線粒體超微結構的影響 [J]. 解剖學報, 2008; 39(6): 854-857.
8. 孔連寶, 吳曉峰, 邵立軍, 等. 吻合肝動脈對大鼠部分肝移植早期損傷的影響 [J]. 中華實驗外科雜志, 2006; 23(5): 543-545.
9. 彭方興, 鄭本波, 劉立新, 等. 大鼠原位肝移植動物模型制作要點 [J]. 中國普外基礎與臨床雜志, 2005; 12(2）: 117-119.
10. 李勇, 張培建. 肝移植術后免疫排斥反應和肝功能監測 [J]. 醫學研究生學報, 2008； 21(3): 313-316.
11. 易述紅, 陳規劃, 陸敏強, 等. VEGF mRNA在預測肝癌肝移植術后腫瘤復發和轉移中的應用價值 [J]. 中國普外基礎與臨床雜志, 2006; 13(5): 560-564.
12. Christou H, Yoshida A, Arthur V, et al． Increased vascular endothelial growth factor production in the lungs of rats with hypoxia-induced pulmonary hypertension [J]. Am J Respir Cell Mol Biol, 1998; 18(6): 768-776.
13. Liu LX, Lu H, Luo Y, et al. Stabilization of vascular endothelial growth factor mRNA by hypoxia-inducible factor 1 [J]. Biochem Biophys Res Commum, 2002; 291(4): 908-914.
14. 李興睿, 廖曉峰, 易繼林. 缺氧對肝癌細胞系HepG2表達VEGF的影響 [J]. 中國普外基礎與臨床雜志, 2005; 12(5): 477-479.
15. Shibuya M, Claesson-Welsh L. Signal transduction by VEGF receptors in regulation of angiogenesis and lymphangiogenesis [J]. Exp Cell Res, 2006; 312(5): 549-560.
16. Wilkinson-Berka JL. Vasoactive factors and diabetic retinopathy: vascular endothelial growth factor, cycoloxygenase-2 and nitric oxide [J]. Curr Pharm Des, 2004; 10(27): 3331-3348.
17. Teraoka H, Sawada T, Nishihara T, et al. Enhanced VEGF production and decreased immunogenicity induced by TGF beta1 promote liver metastasis of pancreatic cancer [J]. Br J Cancer, 2001; 85(4): 612-617.
18. Zuo S, Ji Y, Wang J, et al. Expression and clinical implication of HIF-1alpha and VEGF-C in non-small cell lung cancer [J]. J Huazhong Univ Sci Technolog Med Sci, 2008; 28(6): 674-676.
19. Gaudio E, Barbaro B, Alvaro D, et al. Vascular endothelial growth factor stimulates rat cholangiocyte proliferation via an autocrine mechanism [J]. Gastroenterology, 2006; 130(4): 1270-1282.
20. Gaudio E, Barbara Bo, Alvaro D, et al. Administration of r-VEGF-A prevents hepatic artery ligation-induced bile duct damage in bile duct ligated rats [J]. Am J Physiol Gastrointest Liver Physiol, 2006; 291(2): G307-G317.
21. Xu WH, Ye QF, Xia SS. Apoptosis and proliferation of intrahepatic bile duct after ischemia-reperfusion injury [J]. Hepatobiliary Pancreat Dis Int, 2004; 3(3): 428-432.
22. 李文崗, 胡素賢, 段偉東, 等. 完全失去動脈血供后大鼠肝門部膽管微血管數對膽管上皮細胞核增殖與ER表達影響 [J]. 中華肝膽外科雜志, 2007; 13(8): 548-550.
23. Gaudio E, Onori P, Franchitto A, et al. Hepatic microcirculation and cholangiocyte physiopathology [J]. Ital J Anat Embryol, 2005; 110(2 Suppl 1): 71-75.
24. Bissinger AL, Oettle H, Jahn G, et al. Cytomegalovirus infection after orthotopic liver transplantation is restricted by a pre-existing antiviral immune response of the recipient [J]. J Med Virol, 2004; 73(1): 45-53.
25. Beaussier M, Wendum D, Fouassier L, et al. Adaptative bile duct proliferative response in experimental bile duct ischemia [J]. J Hepatol, 2005; 42(2): 257-265.

《中國普外基礎與臨床雜志》

VEGF參與低氧預適應對移植肝肝內膽管上皮細胞的保護作用

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《中國普外基礎與臨床雜志》

VEGF參與低氧預適應對移植肝肝內膽管上皮細胞的保護作用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

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Content

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