c-Jun基因RNA干擾對大鼠血管平滑肌細胞增殖的影響_《中國普外基礎與臨床雜志》

作者：

張宏偉 ¹ , 周昱 ² , 胡新華 ³ , 張強 ³

1.佳木斯大學附屬第一醫院普通外科(黑龍江佳木斯 154001);;
2.佳木斯大學附屬第一醫院腫瘤科(黑龍江佳木斯 154001);;
3.中國醫科大學附屬第一醫院血管外科（沈陽 110001）;

關鍵詞：

c-Jun基因 RNA干擾血管平滑肌細胞細胞增殖內膜增生激活劑蛋白-1

視頻：

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目的　以RNA干擾抑制血管平滑肌細胞(vascular smooth muscle cells,VSMCs)的c-Jun基因表達,探討其表達抑制后VSMCs增殖的變化。
方法　設對照組（VSMCs不作處理）、陰性siRNA組（VSMCs轉染無關序列的siRNA）及c-Jun siRNA組（VSMCs轉染c-Jun siRNA）。應用RT-PCR半定量法檢測VSMCs中c-Jun的mRNA水平,Western blot法檢測VSMCs中c-Jun的蛋白水平，應用MTT比色法及3H-TdR摻入法檢測VSMCs的增殖情況，流式細胞儀檢測VSMCs的細胞周期變化。
結果　c-Jun siRNA組的c-Jun mRNA及蛋白表達水平均較對照組明顯降低（P＜0.05，P＜0.01），而陰性siRNA組與對照組間差異無統計學意義（P gt;0.05）。c-Jun siRNA組VSMCs的增殖活性較對照組明顯降低（P＜0.05），細胞周期出現明顯的G0／G1期阻滯；而陰性siRNA組與對照組間差異無統計學意義（P gt;0.05）。
結論　RNA干擾介導的c-Jun基因沉默可顯著抑制VSMCs的體外增殖。

引用本文： 張宏偉,周昱,胡新華,張強. c-Jun基因RNA干擾對大鼠血管平滑肌細胞增殖的影響. 中國普外基礎與臨床雜志, 2009, 16(9): 723-728. doi: 復制

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3.	Bulkley BH, Hutchins GM. Accelerated “atherosclerosis”. A morphologic study of 97 saphenous vein coronary artery bypass grafts [Ｊ]. Circulation, 1977; 55(1): 163-169.
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12.	崔廣暉, 廖崇先，陳以旺. 經皮冠狀動脈腔內成形術后再狹窄基因治療現狀與進展 [Ｊ]. 國外醫學內科學分冊, 2005; 32(10): 419-421.
13.	高正山，王樹祥，李慧芳．綜合干預療法冠狀動脈成形術加支架患者的遠期療效 [Ｊ]. 中國臨床康復， 2002; 6(9)： 1257-1258.
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15.	Hiroi N, Nestler EJ. Nuclear memory: gene transcription and behavior [Ｊ]. Adv Pharmacol, 1998; 42: 1037-1041.
16.	Chinenov Y, Kerppola TK. Close encounters of many kinds: Fos-Jun interactions that mediate transcription regulatory specificity [Ｊ]. Oncogene, 2001; 20(19): 2438-2452.
17.	Faries PL, Marin ML, Veith FJ, et al. Immunolocalization and temporal distribution of cytokine expression during the development of vein graft intimal hyperplasia in an experimental model [Ｊ]. J Vasc Surg, 1996; 24(3): 463-471.
18.	何慶南，易著文. 轉錄因子AP-1的研究進展 [Ｊ]. 國外醫學兒科學分冊， 1999； 26（3）： 152-155.
19.	Caplen NJ, Parrish S, Imani F, et al. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems [Ｊ]. Proc Natl Acad Sci USA, 2001; 98(17): 9742-9747.
20.	Elbashir SM, Harborth J, Lendeckel W, et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [Ｊ]. Nature, 2001; 411(6836): 494-498.
21.	韓麗娜，顧瑛，劉凡光，等. c-jun反義核酸對自體移植靜脈內膜增生和ET-1水平的影響 [Ｊ]. 放射免疫學雜志， 2004；17(6)： 429-431.
22.	Angel P, Karin M. The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation [Ｊ]. Biochim Biophys Acta, 1991; 1072(2-3): 129-157.
23.	周敬,　刁清,　王虹. 激活劑蛋白-1誘騙性寡核苷酸對大鼠頸總動脈損傷后新生內膜的影響 [Ｊ]. 疑難病雜志, 2006； 5(3): 164-166.
24.	Kume M, Komori K, Matsumoto T, et al. Administration of a decoy against the activator protein-1 binding site suppresses neointimal thickening in rabbit balloon-injured arteries [Ｊ]. Circulation, 2002; 105(10): 1226-1232.
25.	Gong H, Liu CM, Liu DP, et al. The role of small RNAs in human diseases: potential troublemaker and therapeutic tools [Ｊ]. Med Res Rev, 2005; 25(3): 361-381.

1. Davies MG, Hagen PO. Pathophysiology of vein graft failure: a review [Ｊ]. Eur J Vasc Endovasc Surg, 1995; 9(1): 7-18.
2. Ip JH, Fuster V, Badimon L, et al. Syndromes of accelerated atherosclerosis: role of vascular injury and smooth muscle cell proliferation [Ｊ]. J Am Coll Cardiol, 1990; 15(7): 1667-1687.
3. Bulkley BH, Hutchins GM. Accelerated “atherosclerosis”. A morphologic study of 97 saphenous vein coronary artery bypass grafts [Ｊ]. Circulation, 1977; 55(1): 163-169.
4. 鄭江華, 劉玉娟,　時德. 血管再狹窄的分子生物學研究進展 [Ｊ]. 中國普外基礎與臨床雜志, 2004; 11（4）： 318-321.
5. 張震, 惠汝太. 轉錄因子AP-1與心血管疾病 [Ｊ]. 中國分子心臟病學雜志, 2003; 3（4）: 198-201.
6. Fire A, Xu S, Montgomery MK, et al．Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans [Ｊ]． Nature, 1998; 391(6669): 806-811.
7. Bernstein E, Caudy AA, Hammond SM, et al. Role for a bidentate ribonuclease in the initiation step of RNA interference [Ｊ]. Nature, 2001; 409(6818): 363-366.
8. 周曉莉, 雷寒, 柳青. 小鼠主動脈平滑肌細胞的培養 [Ｊ]. 基礎醫學與臨床, 2005; 25(6): 564-567.
9. Campbell JH, Campbell GR．Culture techniques and their applications to studies of vascular smooth muscle [Ｊ]. Clin Sci (Lond), 1993; 85(5): 501-513.
10. Biswas N, Sanchez V, Spector DH. Human cytomegalovirus infection leads to accumulation of geminin and inhibition of the licensing of cellular DNA replication [Ｊ]. J Virol, 2003; 77(4): 2369-2376.
11. 蔣米爾, 趙海光. 血管外科實驗研究的進展與發展方向 [Ｊ]. 中華實驗外科雜志, 2005; 22(6): 648-650.
12. 崔廣暉, 廖崇先，陳以旺. 經皮冠狀動脈腔內成形術后再狹窄基因治療現狀與進展 [Ｊ]. 國外醫學內科學分冊, 2005; 32(10): 419-421.
13. 高正山，王樹祥，李慧芳．綜合干預療法冠狀動脈成形術加支架患者的遠期療效 [Ｊ]. 中國臨床康復， 2002; 6(9)： 1257-1258.
14. Adcock IM. Transcription factors as activators of gene transcription: AP-1 and NF-kappa B [Ｊ]. Monaldi Arch Chest Dis, 1997; 52(2): 178-186.
15. Hiroi N, Nestler EJ. Nuclear memory: gene transcription and behavior [Ｊ]. Adv Pharmacol, 1998; 42: 1037-1041.
16. Chinenov Y, Kerppola TK. Close encounters of many kinds: Fos-Jun interactions that mediate transcription regulatory specificity [Ｊ]. Oncogene, 2001; 20(19): 2438-2452.
17. Faries PL, Marin ML, Veith FJ, et al. Immunolocalization and temporal distribution of cytokine expression during the development of vein graft intimal hyperplasia in an experimental model [Ｊ]. J Vasc Surg, 1996; 24(3): 463-471.
18. 何慶南，易著文. 轉錄因子AP-1的研究進展 [Ｊ]. 國外醫學兒科學分冊， 1999； 26（3）： 152-155.
19. Caplen NJ, Parrish S, Imani F, et al. Specific inhibition of gene expression by small double-stranded RNAs in invertebrate and vertebrate systems [Ｊ]. Proc Natl Acad Sci USA, 2001; 98(17): 9742-9747.
20. Elbashir SM, Harborth J, Lendeckel W, et al. Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells [Ｊ]. Nature, 2001; 411(6836): 494-498.
21. 韓麗娜，顧瑛，劉凡光，等. c-jun反義核酸對自體移植靜脈內膜增生和ET-1水平的影響 [Ｊ]. 放射免疫學雜志， 2004；17(6)： 429-431.
22. Angel P, Karin M. The role of Jun, Fos and the AP-1 complex in cell-proliferation and transformation [Ｊ]. Biochim Biophys Acta, 1991; 1072(2-3): 129-157.
23. 周敬,　刁清,　王虹. 激活劑蛋白-1誘騙性寡核苷酸對大鼠頸總動脈損傷后新生內膜的影響 [Ｊ]. 疑難病雜志, 2006； 5(3): 164-166.
24. Kume M, Komori K, Matsumoto T, et al. Administration of a decoy against the activator protein-1 binding site suppresses neointimal thickening in rabbit balloon-injured arteries [Ｊ]. Circulation, 2002; 105(10): 1226-1232.
25. Gong H, Liu CM, Liu DP, et al. The role of small RNAs in human diseases: potential troublemaker and therapeutic tools [Ｊ]. Med Res Rev, 2005; 25(3): 361-381.

《中國普外基礎與臨床雜志》

c-Jun基因RNA干擾對大鼠血管平滑肌細胞增殖的影響

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《中國普外基礎與臨床雜志》

c-Jun基因RNA干擾對大鼠血管平滑肌細胞增殖的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

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