大豆胰蛋白酶抑制劑對大鼠胰島分離純化的影響_《中國普外基礎與臨床雜志》

作者：

楊永生 , 張學文 , 張丹 , 孟子輝 , 孫寶震 , 趙吉生 , 王巍

吉林大學中日聯誼醫院肝膽胰外科(長春130033);

關鍵詞：

大豆胰蛋白酶抑制劑胰島分離胰島移植

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的探討大豆胰蛋白酶抑制劑（STI）在大鼠胰島分離純化中對胰島產量及功能的影響。方法按膠原酶中是否加入STI將大鼠分為實驗組和對照組，實驗組在膠原酶消化液中按2.0 mg/ml加入STI，對照組不添加STI。2組均運用膠原酶原位灌注大鼠胰腺的方法來分離胰島，使用Ficoll-400用非連續梯度離心法純化胰島，將純化前、后獲得的胰島進行計數，并對純化后的胰島進行形態、功能檢查。同時進行大鼠同種異體胰島移植觀察其體內功能。結果實驗組和對照組在消化后純化前所得胰島數量無明顯差別〔（624±38.2） IEQ vs （586±37.7） IEQ， P＞0.05〕；在純化后實驗組與對照組所得胰島數量〔（408±28.3） IEQ vs （189±27.1） IEQ，P＜0.05〕和純度〔（93±2.4）% vs （75±2.1）%, P＜0.05〕差異有統計學意義。實驗組與對照組最終所得胰島的體外功能差異無統計學意義（P gt;0.05），體內功能實驗結果差異亦無統計學意義（P gt;0.05)。結論使用在膠原酶中加入STI的消化液原位灌注大鼠胰腺，可以明顯提高大鼠胰島的最終產量和純度，但對胰島的功能無明顯影響。

引用本文： 楊永生,張學文,張丹,孟子輝,孫寶震,趙吉生,王巍. 大豆胰蛋白酶抑制劑對大鼠胰島分離純化的影響. 中國普外基礎與臨床雜志, 2008, 15(11): 813-816. doi: 復制

1.	Ricordi C, Lakey JR, Hering BJ. Challenges toward standardization of islet isolation technology[J]. Transplant Proc, 2001; 33(1-2)∶1709.
2.	London NJ, Thirdborough SM, Loftus IM， et al. Renal subcapsular islet transplantation in the rat: a comparison of three techniques[J]. Diabetes Res, 1991; 16(2)∶81.
3.	Nagata N, Gu Y, Hori H, et al. Evaluation of insulin secretion of isolated rat islets cultured in extracellular matrix[J].Cell Transplant, 2001; 10(4-5)∶447.
4.	Avila JG, Wang Y, Barbaro B, et al. Improved outcomes in islet isolation and transplantation by the use of a novel hemoglobin-based O2 carrier[J]. Am J Transplant, 2006; 6(12)∶2861.
5.	Xiaohui T, Wujun X, Xiaoming D, et al. Small intestinal submucosa improves islet survival and function in vitro culture[J]. Transplant Proc, 2006; 38(5)∶1552.
6.	White SA, Hughes DP, Contractor HH, et al. An investigation into the distribution of different collagen types within adult and juvenile porcine pancreata[J]. J Mol Med, 1999; 77(1)∶79.
7.	White SA, Djaballah H, Hughes DP， et al. A preliminary study of the activation of endogenous pancreatic exocrine enzymes during automated porcine islet isolation[J]. Cell Transplant, 1999; 8(3)∶265.
8.	Heiser A, Ulrichs K, Müller-Ruchholtz W. Isolation of porcine pancreatic islets: low trypsin activity during the isolation procedure guarantees reproducible high islet yields[J]. J Clin Lab Anal, 1994; 8(6)∶407.
9.	Basir I, van der Burg MP, Scheringa M, et al. Improved outcome of pig islet isolation by Pefabloc inhibition of trypsin[J].Transplant Proc, 1997; 29(4)∶1939.
10.	Traverso LW, Abu-Zamzam AM. Activation of pancreatic proteolytic enzymes by commercial collagenases[J].Transplantation, 1978; 25(4)∶226.
11.	姚磊，吳德全，李國東，等. 烏司他丁在大鼠胰島分離過程中的保護作用 [J]. 中華器官移植雜志， 2005; 26（12）∶732.
12.	白日星，宋茂民. 預防性添加胰蛋白酶抑制劑對成年豬胰島分離的影響 [J]. 中國普外基礎與臨床雜志, 2005; 12（3）∶275.
13.	Lakey JR, Helms LM, Kin T, et al. Serine-protease inhibition during islet isolation increases islet yield from human pancreases with prolonged ischemia[J]. Transplantation, 2001; 72(4)∶565.
14.	袁勤生主編. 現代酶學 [M]. 上海: 華東理工大學出版社， 2001∶107~119.
15.	Salvalaggio PR, Deng S, Ariyan CE, et al. Islet filtration: a simple and rapid new purification procedure that avoids ficoll and improves islet mass and function[J].Transplantation, 2002; 74(6)∶877.
16.	張劍, 陳規劃, 翁建平, 等. 抗CD40L單克隆抗體在異種胰島移植排斥反應中的作用 [J]. 中國普外基礎與臨床雜志, 2008; 15(5)∶329.

1. Ricordi C, Lakey JR, Hering BJ. Challenges toward standardization of islet isolation technology[J]. Transplant Proc, 2001; 33(1-2)∶1709.
2. London NJ, Thirdborough SM, Loftus IM， et al. Renal subcapsular islet transplantation in the rat: a comparison of three techniques[J]. Diabetes Res, 1991; 16(2)∶81.
3. Nagata N, Gu Y, Hori H, et al. Evaluation of insulin secretion of isolated rat islets cultured in extracellular matrix[J].Cell Transplant, 2001; 10(4-5)∶447.
4. Avila JG, Wang Y, Barbaro B, et al. Improved outcomes in islet isolation and transplantation by the use of a novel hemoglobin-based O2 carrier[J]. Am J Transplant, 2006; 6(12)∶2861.
5. Xiaohui T, Wujun X, Xiaoming D, et al. Small intestinal submucosa improves islet survival and function in vitro culture[J]. Transplant Proc, 2006; 38(5)∶1552.
6. White SA, Hughes DP, Contractor HH, et al. An investigation into the distribution of different collagen types within adult and juvenile porcine pancreata[J]. J Mol Med, 1999; 77(1)∶79.
7. White SA, Djaballah H, Hughes DP， et al. A preliminary study of the activation of endogenous pancreatic exocrine enzymes during automated porcine islet isolation[J]. Cell Transplant, 1999; 8(3)∶265.
8. Heiser A, Ulrichs K, Müller-Ruchholtz W. Isolation of porcine pancreatic islets: low trypsin activity during the isolation procedure guarantees reproducible high islet yields[J]. J Clin Lab Anal, 1994; 8(6)∶407.
9. Basir I, van der Burg MP, Scheringa M, et al. Improved outcome of pig islet isolation by Pefabloc inhibition of trypsin[J].Transplant Proc, 1997; 29(4)∶1939.
10. Traverso LW, Abu-Zamzam AM. Activation of pancreatic proteolytic enzymes by commercial collagenases[J].Transplantation, 1978; 25(4)∶226.
11. 姚磊，吳德全，李國東，等. 烏司他丁在大鼠胰島分離過程中的保護作用 [J]. 中華器官移植雜志， 2005; 26（12）∶732.
12. 白日星，宋茂民. 預防性添加胰蛋白酶抑制劑對成年豬胰島分離的影響 [J]. 中國普外基礎與臨床雜志, 2005; 12（3）∶275.
13. Lakey JR, Helms LM, Kin T, et al. Serine-protease inhibition during islet isolation increases islet yield from human pancreases with prolonged ischemia[J]. Transplantation, 2001; 72(4)∶565.
14. 袁勤生主編. 現代酶學 [M]. 上海: 華東理工大學出版社， 2001∶107~119.
15. Salvalaggio PR, Deng S, Ariyan CE, et al. Islet filtration: a simple and rapid new purification procedure that avoids ficoll and improves islet mass and function[J].Transplantation, 2002; 74(6)∶877.
16. 張劍, 陳規劃, 翁建平, 等. 抗CD40L單克隆抗體在異種胰島移植排斥反應中的作用 [J]. 中國普外基礎與臨床雜志, 2008; 15(5)∶329.

《中國普外基礎與臨床雜志》

大豆胰蛋白酶抑制劑對大鼠胰島分離純化的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

大豆胰蛋白酶抑制劑對大鼠胰島分離純化的影響

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料