乳腺癌組織中缺氧誘導因子-1α的表達及其與增殖細胞核抗原、微血管密度的關系_《中國普外基礎與臨床雜志》

作者：

郝朗松 ¹ , 朱霞 ² , 錢昆 ¹ , 張清華 ¹ , 羅婷 ¹ , 李小軍 ¹ , 王耕 ² , 伍曉汀 ¹

1.四川大學華西醫院普外科(成都610041);;
2.鄖陽醫學院附屬太和醫院外科(湖北十堰442000);

關鍵詞：

乳腺癌缺氧誘導因子-1α 增殖細胞核抗原微血管密度

視頻：

導出 下載 收藏 掃碼 引用

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

目的　探討乳腺癌組織中缺氧誘導因子-1α（HIF-1α）的表達及其與增殖細胞核抗原（PCNA）、微血管密度（MVD）及臨床病理因素的關系。
方法　采用免疫組化SP法檢測乳腺纖維腺瘤、普通乳腺增生組織和乳腺癌組織中HIF-1α及PCNA蛋白的表達，用CD34單克隆抗體標記血管內皮細胞并計數MVD。
結果　HIF-1α蛋白在乳腺纖維腺瘤、普通乳腺增生組織中不表達；在乳腺導管內原位癌中表達陽性率為55.0%（11/20），浸潤性乳腺癌中為85.0%（51/60）； HIF-1α表達與淋巴結轉移及雌激素受體狀態有關(P＜0.01)。乳腺癌中PCNA高表達率為75.0%（60/80），其中導管內原位癌中為65.0%（13/20），浸潤性癌中為78.3%（47/60）。乳腺癌中HIF-1α表達與PCNA呈正相關（r=0.693，P＜0.01）,與導管內原位癌中MVD亦呈正相關(r=0.682，P＜0.05)，與浸潤性癌中MVD無相關關系。
結論　HIF-1α在乳腺癌組織中的表達明顯上調，與腫瘤細胞增殖、血管形成、淋巴結轉移、雌激素受體狀態相關，提示HIF-1α對乳腺癌的腫瘤細胞增殖、血管形成、生長和侵襲、轉移起重要作用，有望成為腫瘤治療的新靶點。

引用本文： 郝朗松,朱霞,錢昆,張清華,羅婷,李小軍,王耕,伍曉汀. 乳腺癌組織中缺氧誘導因子-1α的表達及其與增殖細胞核抗原、微血管密度的關系. 中國普外基礎與臨床雜志, 2007, 14(2): 203-207. doi: 復制

1.	Semenza GL. HIF-1: mediator of physiological and pathophysiological responses to hypoxia [J]. J Appl Physiol， 2000; 88(4)∶1474.
2.	Acker T, Plate KH. A role for hypoxia and hypoxia-inducible transcription factors in tumor physiology [J]. J Mol Med， 2002; 80(9)∶562.
3.	Bos R, van Diest PJ, van der Groep P, et al. Expression of hypoxia-inducible factor-1alpha and cell cycle proteins in invasive breast cancer are estrogen receptor related [J]. Breast Cancer Res， 2004; 6(4)∶R450.
4.	Mommers EC, Poulin N, Meijer CJ, et al. Malignancy-associated changes in breast tissue detected by image cytometry [J]. Anal Cell Pathol， 2000; 20(4)∶187.
5.	Akakura N, Kobayashi M, Horiuchi I, et al. Constitutive expression of hypoxia-inducible factor-1 alpha renders pancreatic cancer cells resistant to apoptosis induced by hypoxia and nutrient deprivation [J]. Cancer Res， 2001; 61(17)∶6548.
6.	Laughner E, Taghavi P, Chiles K, et al. HER2 (neu) signaling increases the rate of hypoxia-inducible factor 1 alpha (HIF-1 alpha) synthesis: novel mechanism for HIF-1-mediated vascular endothelial growth factor expression [J]. Mol Cell Biol， 2001; 21(12)∶3995.
7.	Zhong H, Chiles K, Feldser D, et al. Modulation of hypoxia-inducible factor 1 alpha expression by the epidermal growth factor/phosphatidylinositol 3-kinase/PTEN/AKT/FRAP pathway in human prostate cancer cells: implications for tumor angiogenesis and therapeutics [J]. Cancer Res， 2000; 60(6)∶1541.
8.	Rose F, Grimminger F, Appel J, et al. Hypoxic pulmonary artery fibroblasts trigger proliferation of vascular smooth muscle cells: role of hypoxia-inducible transcription factors [J]. FASEB J， 2002; 16(12)∶1660.
9.	Kung AL, Wang S, Klco JM, et al. Suppression of tumor growth through disruption of hypoxia-inducible transcription [J]. Nat Med， 2000; 6(12)∶1335.
10.	Li L, Lin X, Staver M, et al. Evaluating hypoxia-inducible factor-1alpha as a cancer therapeutic target via inducible RNA interference in vivo [J]. Cancer Res， 2005; 65(16)∶7249.
11.	Moeller BJ, Dreher MR, Rabbani ZN, et al. Pleiotropic effects of HIF-1 blockade on tumor radiosensitivity [J]. Cancer Cell， 2005; 8(2)∶99.

1. 　 Semenza GL. HIF-1: mediator of physiological and pathophysiological responses to hypoxia [J]. J Appl Physiol， 2000; 88(4)∶1474.
2. 　Acker T, Plate KH. A role for hypoxia and hypoxia-inducible transcription factors in tumor physiology [J]. J Mol Med， 2002; 80(9)∶562.
3. 　Bos R, van Diest PJ, van der Groep P, et al. Expression of hypoxia-inducible factor-1alpha and cell cycle proteins in invasive breast cancer are estrogen receptor related [J]. Breast Cancer Res， 2004; 6(4)∶R450.
4. 　Mommers EC, Poulin N, Meijer CJ, et al. Malignancy-associated changes in breast tissue detected by image cytometry [J]. Anal Cell Pathol， 2000; 20(4)∶187.
5. 　Akakura N, Kobayashi M, Horiuchi I, et al. Constitutive expression of hypoxia-inducible factor-1 alpha renders pancreatic cancer cells resistant to apoptosis induced by hypoxia and nutrient deprivation [J]. Cancer Res， 2001; 61(17)∶6548.
6. 　Laughner E, Taghavi P, Chiles K, et al. HER2 (neu) signaling increases the rate of hypoxia-inducible factor 1 alpha (HIF-1 alpha) synthesis: novel mechanism for HIF-1-mediated vascular endothelial growth factor expression [J]. Mol Cell Biol， 2001; 21(12)∶3995.
7. 　Zhong H, Chiles K, Feldser D, et al. Modulation of hypoxia-inducible factor 1 alpha expression by the epidermal growth factor/phosphatidylinositol 3-kinase/PTEN/AKT/FRAP pathway in human prostate cancer cells: implications for tumor angiogenesis and therapeutics [J]. Cancer Res， 2000; 60(6)∶1541.
8. 　Rose F, Grimminger F, Appel J, et al. Hypoxic pulmonary artery fibroblasts trigger proliferation of vascular smooth muscle cells: role of hypoxia-inducible transcription factors [J]. FASEB J， 2002; 16(12)∶1660.
9. 　Kung AL, Wang S, Klco JM, et al. Suppression of tumor growth through disruption of hypoxia-inducible transcription [J]. Nat Med， 2000; 6(12)∶1335.
10. 　Li L, Lin X, Staver M, et al. Evaluating hypoxia-inducible factor-1alpha as a cancer therapeutic target via inducible RNA interference in vivo [J]. Cancer Res， 2005; 65(16)∶7249.
11. 　Moeller BJ, Dreher MR, Rabbani ZN, et al. Pleiotropic effects of HIF-1 blockade on tumor radiosensitivity [J]. Cancer Cell， 2005; 8(2)∶99.

《中國普外基礎與臨床雜志》

乳腺癌組織中缺氧誘導因子-1α的表達及其與增殖細胞核抗原、微血管密度的關系

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

《中國普外基礎與臨床雜志》

乳腺癌組織中缺氧誘導因子-1α的表達及其與增殖細胞核抗原、微血管密度的關系

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料