COX-2與HIF-1α在肝癌中的表達與相關性_《中國普外基礎與臨床雜志》

作者：

姜超 , 張峰 , 王少軍 , 鄒奇飛

南京醫科大學第一附屬醫院肝臟外科(南京 210029);

關鍵詞：

原發性肝癌美洛昔康環氧合酶-2 缺氧誘導因子-1α

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【摘要】目的研究肝癌中環氧合酶-2(COX-2)和缺氧誘導因子-1α(HIF-1α)表達的相關性，探討選擇性COX-2抑制劑抑癌作用的可能機理。
方法通過免疫組化染色檢測53例肝癌標本中COX-2和HIF-1α的表達；利用不同濃度的選擇性COX-2抑制劑美洛昔康對在正常和氯化鈷模擬缺氧條件下培養的肝癌SMMC-7721細胞進行處理，Western blot法分別檢測各組HIF-1α的表達情況。
結果53例標本中COX-2表達強陽性率為41.5%（22/53），表達弱陽性率為20.8%（11/53），表達陰性率為37.7%（20/53）; HIF-1α表達強陽性率為34.0%(18/53），表達弱陽性率為34.0%(18/53)，表達陰性率為32.1%（17/53）。COX-2和HIF-1α的表達呈正相關（r=0.44,P＜0.01）。常氧條件下HIF-1α表達相對值為0.185±0.057，經美洛昔康處理后HIF-1α不表達。缺氧刺激下HIF-1α表達相對值升高至1.011±0.131，而加入美洛昔康后明顯降低（P＜0.05），且隨著美洛昔康濃度的增加，降低越明顯（P＜0.05）。
結論美洛昔康不僅能夠抑制常氧條件下HIF-1α的表達，而且呈濃度依賴性地抑制缺氧條件下HIF-1α表達的上調，選擇性COX-2抑制劑的抑癌作用機理可能部分是由HIF-1α介導的。

引用本文： 姜超,張峰,王少軍,鄒奇飛. COX-2與HIF-1α在肝癌中的表達與相關性. 中國普外基礎與臨床雜志, 2006, 13(2): 142-141. doi: 復制

1.	Huang SP, Wu MS, Shun CT, et al. Cyclooxygenase2 increases hypoxiainducible factor1 and vascular endothelial growth factor to promote angiogenesis in gastric carcinoma [Ｊ]. J Biomed Sci， 2005; 12(1)∶229.
2.	Bae SH, Jung ES, Park YM, et al. Expression of cyclooxygenase2 (COX2) in hepatocellular carcinoma and growth inhibition of hepatoma cell lines by a COX2 inhibitor, NS398 [Ｊ]. Clin Cancer Res， 2001; 7(5)∶ 1410.
3.	Fosslien E. Review: molecular pathology of cyclooxygenase2 in cancerinduced angiogenesis [Ｊ]. Ann Clin Lab Sci, 2001; 31(4)∶325.
4.	Gallo O, Franchi A, Magnelli L, et al. Cyclooxygenase2 pathway correlates with VEGF expression in head and neck cancer. Implications for tumor angiogenesis and metastasis [Ｊ]. Neoplasia, 2001; 3(1)∶53.
5.	Leahy KM, Koki AT, Masferrer JL. Role of cyclooxygenases in angiogenesis [Ｊ]. Curr Med Chem， 2000; 7(11)∶1163.
6.	Semenza GL. Targeting HIF1 for cancer therapy [Ｊ]. Nat Rev Cancer, 2003; 3(10)∶721.
7.	丁磊，陳孝平，王海平. 肝癌和癌旁組織中HIF1α基因蛋白的表達及其臨床意義 [Ｊ]. 肝膽外科雜志， 2004; 12(1)∶32.
8.	Blancher C, Moore JW , Talks KL, et al. Relationship of hypoxiainducible factor (HIF)1alpha and HIF2alpha expression to vascular endothelial growth factor induction and hypoxia survival in human breast cancer cell lines [Ｊ]. Cancer Res， 2000; 60(24)∶710.
9.	Xu XC. COX2 inhibitors in cancer treatment and prevention, a recent development [Ｊ]. Anticancer Drugs， 2002; 13(2)∶127.
10.	Yao M, Kargman S, Lam EC, et al. Inhibition of cyclooxygenase2 by rofecoxib attenuates the growth and metastatic potential of colorectal carcinoma in mice [Ｊ]. Cancer Research, 2003; 63(3)∶ 586.
11.	Abiru S, Nakao K, Ichikawa T, et al. Aspirin and NS398 inhibit hepatocyte growth factorinduced invasiveness of human hepatoma cells [Ｊ]. Hepatology， 2002; 35(5)∶1117.

1. Huang SP, Wu MS, Shun CT, et al. Cyclooxygenase2 increases hypoxiainducible factor1 and vascular endothelial growth factor to promote angiogenesis in gastric carcinoma [Ｊ]. J Biomed Sci， 2005; 12(1)∶229.
2. Bae SH, Jung ES, Park YM, et al. Expression of cyclooxygenase2 (COX2) in hepatocellular carcinoma and growth inhibition of hepatoma cell lines by a COX2 inhibitor, NS398 [Ｊ]. Clin Cancer Res， 2001; 7(5)∶ 1410.
3. Fosslien E. Review: molecular pathology of cyclooxygenase2 in cancerinduced angiogenesis [Ｊ]. Ann Clin Lab Sci, 2001; 31(4)∶325.
4. Gallo O, Franchi A, Magnelli L, et al. Cyclooxygenase2 pathway correlates with VEGF expression in head and neck cancer. Implications for tumor angiogenesis and metastasis [Ｊ]. Neoplasia, 2001; 3(1)∶53.
5. Leahy KM, Koki AT, Masferrer JL. Role of cyclooxygenases in angiogenesis [Ｊ]. Curr Med Chem， 2000; 7(11)∶1163.
6. Semenza GL. Targeting HIF1 for cancer therapy [Ｊ]. Nat Rev Cancer, 2003; 3(10)∶721.
7. 丁磊，陳孝平，王海平. 肝癌和癌旁組織中HIF1α基因蛋白的表達及其臨床意義 [Ｊ]. 肝膽外科雜志， 2004; 12(1)∶32.
8. Blancher C, Moore JW , Talks KL, et al. Relationship of hypoxiainducible factor (HIF)1alpha and HIF2alpha expression to vascular endothelial growth factor induction and hypoxia survival in human breast cancer cell lines [Ｊ]. Cancer Res， 2000; 60(24)∶710.
9. Xu XC. COX2 inhibitors in cancer treatment and prevention, a recent development [Ｊ]. Anticancer Drugs， 2002; 13(2)∶127.
10. Yao M, Kargman S, Lam EC, et al. Inhibition of cyclooxygenase2 by rofecoxib attenuates the growth and metastatic potential of colorectal carcinoma in mice [Ｊ]. Cancer Research, 2003; 63(3)∶ 586.
11. Abiru S, Nakao K, Ichikawa T, et al. Aspirin and NS398 inhibit hepatocyte growth factorinduced invasiveness of human hepatoma cells [Ｊ]. Hepatology， 2002; 35(5)∶1117.

《中國普外基礎與臨床雜志》

COX-2與HIF-1α在肝癌中的表達與相關性

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《中國普外基礎與臨床雜志》

COX-2與HIF-1α在肝癌中的表達與相關性

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料