睪丸免疫豁免細胞的分離及其功能測定_《中國普外基礎與臨床雜志》

作者：

1.華西醫科大學附屬第一醫院外科，現在中山醫科大學附屬第一醫院外科(廣州510080);;
2.中山醫科大學預防保健科(廣州510080);;
3.華西醫科大學法醫學系病理室(成都610041);

關鍵詞：

睪丸細胞免疫豁免 Fas配體

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目的探討睪丸產生免疫豁免的機理。
方法應用膠原酶、胰蛋白酶及脫氧核糖核酸酶消化制備睪丸Sertoli細胞，體外與活性淋巴細胞共同培養，了解其對淋巴細胞的殺傷作用。并用SABC法標記睪丸組織Sertoli細胞Fas配體的表達。
結果 Sertoli細胞可殺傷與之共培養的活性淋巴細胞，睪丸組織Sertoli細胞有明顯的Fas配體表達。
結論 Fas配體陽性的Sertoli細胞對活性淋巴細胞的殺傷作用可能與睪丸免疫豁免部位有關。

引用本文： 蘭平,張梅,官鵬,嚴律南,肖路加. 睪丸免疫豁免細胞的分離及其功能測定. 中國普外基礎與臨床雜志, 2000, 7(2): 83-84下轉. doi: 復制

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1. Medawar PB. Immunity to homologous grafted skin Ⅲ in the fate of skin homografts transplanted to the brain to subcutaneous tissue, and to the anterior 〔Ｊ〕. Br J Exp Pathol, 1948; 29(1)∶58.
2. Neaves WB, Billingham RE. The lymphatic drainage of the rat prostate and its status as an immunologically privileged site 〔Ｊ〕. Transplantation, 1979; 27(1)∶127.
3. Korbutt GS, Elliott JF, Rojotte R. Cotransplantation of allogeneic islets with allogeneic testicular cell aggregates allows longterm graft survival without systemic immunosuppression 〔Ｊ〕. Diabetes, 1997; 46(2)∶317.
4. Griffith TS, Brunner J, Fletcher SM. Fas ligandinduced apoptosis as a mechanism of immune privilege 〔Ｊ〕. Science, 1995; 270(17)∶1189.
5. Selawry HP, Cameron DF. Sertoli cellenriched fractions in successful islet cell transplantation 〔Ｊ〕. Cell Transplantation, 1993; 2(1)∶123.
6. Nagata S, Golstein P. The Fas death factor 〔Ｊ〕. Science, 1995; 267(5203)∶1449.
7. Selaway HP, Kotb M, Herrod HG, et al. Production of a factor, or factors, suppressing IL2 production and Tcell proliferation by sertoli cellenriched preparations: a potention role for islet transplantation in an immunologically privileged site 〔Ｊ〕. Transplantation, 1991; 52(5)∶846.
8. Bellgrau D, Dild D, Selawry H. A role for CD95 ligand in preventing graft rejection 〔Ｊ〕. Nature, 1995; 377(6550)∶630.
9. Selawry HP, Cameron DF. Sertoli cellenriched fractions in successful islet cell transplantation 〔Ｊ〕. Cell Transplantation, 1993; 2∶123.

《中國普外基礎與臨床雜志》

睪丸免疫豁免細胞的分離及其功能測定

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《中國普外基礎與臨床雜志》

睪丸免疫豁免細胞的分離及其功能測定

摘要 全文 圖表 視頻 參考文獻 施引文獻 補充材料

Format

Content

摘要全文圖表視頻參考文獻施引文獻補充材料